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Tick infestations are a recurring roadblock of human development around the world, with estimated damages in the global economic landscape being as high as 30 billion USD. Specifically, India has long been sus…
Read guide →Histone H3K27M is a driving mutation in diffuse intrinsic pontine glioma (DIPG), a deadly pediatric brain tumor. The malignant and treatment-resistant brain tumor is a target for anti-cancer studies using cell…
Read guide →Platinum based anticancer drugs generally go hand-in-hand with DNA, however their interactions with proteins are important to understand as well. A group focused their work on discovering and further exploring…
Read guide →Intraspecies pheromone signaling regulated by proteases is critical for fungi procreation. The fungal aspartyl protease Bar1 was shown to have unique substrate specificity of important implications in fungal e…
Read guide →Lyme disease is an abundantly present vector-born disease, with nearly 400,000 new cases in the U.S. diagnosed each year. While treatment with antibiotics is effective, if the disease goes unnoticed and unchec…
Read guide →LifeTein recently announced a significant accomplishment in peptide synthesis: the successful creation of the C subunit of the ATP synthase. This subunit is a highly hydrophobic peptide composed of 75 residues…
Read guide →A common strategy for nanomaterials to enter the cell has always been covalent coupling with cell-penetrating peptides (CPPs). While effective, it is not always desirable to make chemical modifications to the …
Read guide →Equipped with exceptional cell permeability and a robust affinity for RNA, LifeTein’s Peptide Nucleic Acid (PNA) synthesis service emerges as the premier platform technology, continuously evolving to meet dive…
Read guide →It is highly challenging to deliver cosmetic actives and drugs to the skin because of the stratum corneum (SC). The permeability of large hydrophilic peptides or compounds across the stratum corneum on the out…
Read guide →Blocking the interaction between Programmed Death Ligand 1 (PD-L1) and its receptor, PD-1, is an effective method of treating many types of cancers. Identifying the PD-L1 binding peptide and mock peptide: Sele…
Read guide →Peptides were synthesized using standard Fmoc solid-phase peptide synthesis. After coupling, Lys(Mtt) was selectively deprotected to expose the side-chain amine moiety. Cy3 or Cy5 were dissolved in DMF, activa…
Read guide →Peptide libraries are a cornerstone in the field of biochemical research, offering a versatile tool for various applications ranging from drug discovery to therapeutic development. Understanding the utility an…
Read guide →Expression of human endogenous retroviruses (HERVs) shows potential for peptide derivatives to be used as biomarkers for prostate cancer. Specifically, peptides from HERV-K and HERV-H Proteins show association…
Read guide →Table 1 Selection of cell-penetrating peptides (Reference: https://doi.org/10.1007/978-981-13-8747-0, Ülo Langel, CPP, Cell-Penetrating Peptides, 2019) Name Sequence 2 DSLKSYWYLQKFSWR (Kondo et al. 2012) 18A D…
Read guide →How long do peptides last in the fridge? How should peptides be stored? For long-term storage, the peptide should be kept in solid form in the deep freezer at < -15 °C. If stored at room temperature some pepti…
Read guide →Epitope mapping identifies antigen regions that serve as binding sites for antibodies. The overlapping linear peptides derived from the primary sequence of the antigen are frequently used for epitope screening…
Read guide →Protein-protein interactions (PPIs) are fundamental to virtually all biological processes, ranging from cellular signaling and structural organization to enzymatic activity and immunological recognition. Modul…
Read guide →Labeled peptides are frequently used by researchers for binding studies, to determine substrate specificity, and for receptor cross-linking studies. Many researchers would like to synthesize biotin, FITC, nano…
Read guide →Dimethyl sulfoxide (DMSO) is a polar, aprotic organic solvent widely utilized in cell culture, especially as a cryoprotectant to prevent ice crystal formation during freezing. Its unique membrane-penetrating a…
Read guide →LifeTein Dot Blot protocol # Dot Blot protocol Dot blot is similar to the Western blot technique. However, the proteins are spotted directly onto the membrane or paper for detecting and analyzing. This is a go…
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