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A peptide RK-10 is used to detect Programmed Death Ligand 1 (PD-L1) expressing tumors with immunohistochemistry. In order to detect PD-L1 in tissues, a biotin-conjugated version of peptide RK-10-Cy5 was used. …
Read guide →Purified peptides must be free of Trifluoroacetate (TFA) because TFA could alter the results of downstream biological assays. Synthetic peptides are manufactured using solid-phase procedures. TFA is usually us…
Read guide →Sample Protocol Cell-penetrating peptides (CPPs) have emerged as a versatile tool in molecular biology, particularly for facilitating the transfection of DNA, RNA, and other macromolecules into cells. These pe…
Read guide →1.Mapping of the monoclonal antibody epitopes was performed using linear peptides that were 15 amino acids in length and that overlapped by 11 residues spanning the full length of the peptide. 2.The ELISA plat…
Read guide →Dissolving peptides in dimethyl sulfoxide (DMSO) for use in cell culture experiments requires careful handling to ensure both peptide solubility and cell safety. Here’s a step-by-step guide on how to do it: Ma…
Read guide →The development and application of Multiple Antigenic Peptides (MAPs) in biological research and antibody production mark a significant advancement in the field of immunology and peptide science. MAPs, essenti…
Read guide →The shelf life of reconstituted peptides in the refrigerator can vary depending on several factors, including the nature of the peptide, the storage conditions, and the presence of preservatives. Try fast synt…
Read guide →Peptide FAQ Peptide FAQ # Frequently Asked Peptide Questions How long do peptides last in the fridge? How should peptides be stored? For the long-term storage, the peptide should be kept in solid form in the d…
Read guide →ELISA Protocol # Coating the appropriate antigen to microplate 1. Dilute the antigen to a final concentration of 20 μg/ml in PBS. Fill the microwells of a Nunc Maxi-Sorp Immuno Plate with 50 μL of the diluted …
Read guide →Cell Penetrating Peptides Cell Penetrating Peptides # Cell-penetrating peptides (CPPs) have emerged as revolutionary tools in biomedical research and therapy, particularly for their ability to facilitate the t…
Read guide →Cyclic peptides as broad-spectrum antiviral agents Antiviral drugs and vaccines are the most powerful tools to combat viral diseases. Most drugs and vaccines only target a single virus. However, the broad-spec…
Read guide →Cyclic peptides are macromolecules with restricted structures that have stronger competitive edges than linear biological entities. They have been reported to possess various activities, such as antifungal, an…
Read guide →Peptide Targeting Cancer Cancer is expected to surpass the current number one, cardiovascular diseases by 2030 as the leading cause of death. The targeting peptides can be considered as an alternative vehicle …
Read guide →Cell-penetrating peptides (CPPs) are a promising class of short peptides with the ability to translocate across the cell membrane. In general, CPPs can be divided into three classes: Cationic, amphipathic and …
Read guide →Cell Penetrating Peptides (CPPs), particularly the Trans-Activator of Transcription (TAT, GRKKRRQRRRPQ) from the Human Immunodeficiency Virus (HIV), have garnered significant attention for their ability to tra…
Read guide →BRAF is the most frequently mutated kinase in human cancers and is one of the major effectors of oncogenic RAS. So BRAF is a target of interest for anti-cancer drug development and peptide inhibitors. Two FDA-…
Read guide →Cell-penetrating peptides (CPPs) are a class of short peptides that have the ability to facilitate the intracellular delivery of various molecular cargos. Among these, R9, a peptide consisting of nine arginine…
Read guide →Zonula occludens toxin (Zot) and its biologically active fragment, delta G, have been shown to reversibly open tight junctions (TJ) in endothelial and epithelial cells. AT1002, a six-mer synthetic peptide H-FC…
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