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Highlights • Early large α-synuclein aggregates populate the solutions. • Interpretation of α-synuclein UV absorption spectra. • Label-free analysis of α-synuclein aggregation. • Stabilization of α-synuclein b…
Read guide →November 1, 2012 (Vol. 32, No. 19) New System Focuses on Intrinsic Amide Bonds Standard methods for protein quantitation rely on colorimetric assays like those involving protein-copper chelation (BCA and Lowry…
Read guide →Ultraviolet absorption spectroscopy of proteins Proteins, such as those in animal tissue and plants, strongly absorb ultraviolet (UV) light at approximately 280 nm. Rather, it is some of the amino acids which …
Read guide →Springer Protocols (2014) Authors: Mangala R. Liyanage 1 , Kunal Bakshi 1 , David B. Volkin 1 , C. Russell Middaugh 1 Mangala R. Liyanage 1 , Kunal Bakshi 1 , David B. Volkin 1 , C. Russell Middaugh 1 Protocol…
Read guide →Contents Why Measure Peptide Concentration? The Beer-Lambert Law 280 nm Method: Aromatic Amino Acids 214 nm Method: Peptide Bonds Interactive Calculators Worked Examples Troubleshooting & Tips References Why M…
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