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Editorial team for Peptide Therapy Guide.
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Blocking the interaction between Programmed Death Ligand 1 (PD-L1) and its receptor, PD-1, is an effective method of treating many types of cancers. Identifying the PD-L1 binding peptide and mock peptide: Sele…
Read guide →Please view this video on how to design cell penetrating peptides. The transcript is listed below. Transcript Slide 1: Thank you for joining me. My topic today is the cell-penetrating peptides. My main focus w…
Read guide →Peptides were synthesized using standard Fmoc solid-phase peptide synthesis. After coupling, Lys(Mtt) was selectively deprotected to expose the side-chain amine moiety. Cy3 or Cy5 were dissolved in DMF, activa…
Read guide →Aβ-(1–42) was dissolved to 1 mM in 100% hexafluoroisopropanol, hexafluoroisopropanol was removed under vacuum, and the peptide was stored at −20 °C. For the aggregation protocols, the peptide was first resuspe…
Read guide →Peptide libraries are a cornerstone in the field of biochemical research, offering a versatile tool for various applications ranging from drug discovery to therapeutic development. Understanding the utility an…
Read guide →Cells were seeded on 96-well culture plates (10000 cells/well) and incubated in 100 μL of DMEM containing 10% FBS. The medium was then replaced with fresh medium containing 10% FBS, and a peptide solution was …
Read guide →The anti-apoptotic factor Bcl-2 is over-expressed in B-cell lymphoma cells as their primary survival mechanism by binding to IP3R2 on the endoplasmic reticulum (ER). In this study, a cell-penetrating version o…
Read guide →Short Peptide Folding How does the amino acid sequence of a protein chain determine and maintain its 3D folded state? How do small proteins fold? Short Peptide Folding Many small proteins or miniproteins are p…
Read guide →LifeTein FRET peptide helped researchers identify a small molecule drug candidate, DMA-135, against Enterovirus 71 (EV71), a virus that causes foot-to-mouth disease with no FDA approved drugs. The Tat-derived …
Read guide →Enhancing the water solubility of peptide sequences is a critical aspect of peptide-based therapeutic development and biochemical research. This article explores various strategies and scientific insights into…
Read guide →As the world moves on through the ongoing pandemic that is COVID-19, great minds across every field and corner of the world are doing their part to further our collective understanding of the virus. A recent s…
Read guide →Expression of human endogenous retroviruses (HERVs) shows potential for peptide derivatives to be used as biomarkers for prostate cancer. Specifically, peptides from HERV-K and HERV-H Proteins show association…
Read guide →Table 1 Selection of cell-penetrating peptides (Reference: https://doi.org/10.1007/978-981-13-8747-0, Ülo Langel, CPP, Cell-Penetrating Peptides, 2019) Name Sequence 2 DSLKSYWYLQKFSWR (Kondo et al. 2012) 18A D…
Read guide →Alzheimer’s disease as we know it today is a horrible and currently incurable neurodegenerative disorder characterized by neuronal loss, memory impairment, and cognitive decline. Ongoing research is always loo…
Read guide →Fluorescent peptide labelling with Cy5, a cyanine dye, has become an indispensable technique in biomedical research, enabling the precise visualization and tracking of peptides in complex biological systems. T…
Read guide →How long do peptides last in the fridge? How should peptides be stored? For long-term storage, the peptide should be kept in solid form in the deep freezer at < -15 °C. If stored at room temperature some pepti…
Read guide →Fluorescent labelling is a cornerstone technique for visualizing and quantifying biomolecular interactions, with 7-Methoxycoumarin-4-acetic acid (MCA) standing out as a particularly versatile fluorophore for p…
Read guide →Fluorescent labeling with Cy5.5 has become a cornerstone in biomedical research, enabling the visualization and tracking of biological molecules in complex environments. This article delves into the specifics …
Read guide →Fluorescent labeling is a powerful technique used in cell biology and microscopy to visualize specific molecules within cells. Among the various fluorophores available, Texas Red stands out as a bright red-flu…
Read guide →Fluorescent labeling with FAM (carboxyfluorescein) has become a foundational technique for visualizing and tracking peptides in a wide range of biological systems. This bright, green fluorophore is prized for …
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