Educational guide
Revolutionizing mRNA Concentration Measurement with Variable Pathlength Spectroscopy
Messenger ribonucleic acid (mRNA) is a single-stranded molecule of RNA that carries the instructions from DNA to create specific proteins. The main applications for mRNA include immunotherapy, protein replacement therapy, genvomic editing, and regenerative med
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The CTech™ SoloVPER System uses a variable pathlength method to measure concentration, with a light scattering correction algorithm to address the issue presented by scattering from LNPs. This publication demonstrates how the SoloVPE System was used to measure the total mRNA concentration after LNP encapsulation. The method was developed and qualified by assessing the specificity, repeatability, intermediate precision, linearity, and accuracy of the concentration measurements. The direct measurement of mRNA content using the CTech SoloVPE System passed all acceptance criteria, demonstrating the reliability of the variable pathlength method.