PerCP

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Excitation & emission spectra

Key facts

Excitation maximum 482 nm Emission maximum 675 nm Optimal laser line 488 Optimal filter 670/30 Relative brightness 1 of 5 Extinction coefficient 350000 Molecular weight 35000 Commercial conjugates 1.4k from 40 suppliers

PerCP has an excitation maximum at 482 nm and an emission maximum at 675 nm, a Stokes shift of 193 nm. Its extinction coefficient is 350000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.

Frequently asked questions

What is PerCP? Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter. PerCP exhibits a large Stokes' Shift, with an excitation peak at 482 nm and an emission peak at 675 nm. PerCP is was historically used in flow cytometry, however it is highly susceptible to photobleaching and has poor stability. Alternatives like BB700, NovaFluor Blue 690 or PerCP-eFluor™ 710 are preferred. PerCP is a generic dye that has no sole manufacturer. What laser excites PerCP? PerCP is optimally excited by the 488 laser line. Its excitation maximum is 482 nm. What filter should I use to detect PerCP? A 670/30 filter captures the peak of the PerCP emission near 675 nm with minimal spillover. How bright is PerCP? PerCP has a relative brightness of 1 out of 5. How many antibodies are available conjugated to PerCP? FluoroFinder tracks 1430 commercially available PerCP conjugates, searchable by target, clone, host and application.