FcIII peptide

Product Information

• Product Name

  FcIII peptide

• Documents
• Sequence Shortening

  H-DCAWHLGELVWCT-OH (C2-C12 disulfide bridge)

• Sequence

  H-Asp-Cys-Ala-Trp-His-Leu-Gly-Glu-Leu-Val-Trp-Cys-Thr-OH (C2-C12 disulfide bridge)

• Length (aa)

  13

• Peptide Purity (HPLC)

  97.22%

• Molecular Formula

  C69H95N17O19S2

• Molecular Weight

  1530.75

• Source

  Synthetic

• Form

  Powder

• Description

  The FcIII peptide is a 13 amino acid peptide (DCAWHLGELVWCT) that has been shown to bind competitively to hinge region on the Fc fragment of IgG with high affinity. It was initially identified through a bacteriophage display screen. A modified version of the FcIII peptide, called Bio-Ox, was developed to increase solubility and enable competitive elution of protein complexes in their native state. Further modifications were made to improve solubility, resulting in a new reagent called PEGylOx. PEGylOx is an FcIII peptide that is N-terminally PEGylated with a four-unit polymer and cyclized by oxidation of the cysteines to cystine. PEGylOx has been used for the rapid, native elution of protein complexes containing a Protein-A-tagged component from yeast and bacteria.

  There is another modified peptide,Fc-III-4C (Cat.# 319362), H-CDCAWHLGELVWCTC-OH, (C1-C15, C3-C13 disulfide bridges). The binding affinity of Fc–III–4C tag to human IgG is measured as 2.28 nM (Kd) and is 100 times higher than that of the Fc-III tag to IgG.

  The primary sequence of Fc-III peptide was DCAWHLGELVWCT, in which Cys 2 and Cys 12 form a disulfide linkage that make the peptide has a cyclic structure.

• Storage Guidelines

  Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• References
  • LaCava J, Chandramouli N, Jiang H, Rout MP. Improved native isolation of endogenous Protein A-tagged protein complexes. Biotechniques. 2013 Apr;54(4):213-6. doi: 10.2144/000114012. PMID: 23581468; PMCID: PMC3667664.
  • Gong Y, Yi M, Zhang L, Feng S, Deng H. Characterization of the Fc-III-4C-based recombinant protein expression system by using carbonic anhydrase as the model protein. Protein Expr Purif. 2021 Jan;177:105761. doi: 10.1016/j.pep.2020.105761. Epub 2020 Sep 19. PMID: 32956801.
• About TFA salt

  Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

  Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

  Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

  In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

• Molar Concentration Calculator

• Dilution Calculator

• Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.