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Be on the Look-out: Dive Deep into the World of PepMix

Be on the Look-out this Fall: Dive Deep into the World of PepMix™ Peptide Pools! Published on 10/09/2024 PepMix™ peptide pools are overlapping peptides spanning a whole protein or antigen, and the industry gold standard for antigen-specific T cell stimulation.

Written by Peptide Therapy Guide Editorial Team
For education only

This guide cannot diagnose a condition or recommend a personal treatment plan. Discuss medical questions with a qualified professional.

Be on the Look-out this Fall: Dive Deep into the World of PepMix™ Peptide Pools!

Published on 10/09/2024

PepMix™ peptide pools are overlapping peptides spanning a whole protein or antigen, and the industry gold standard for antigen-specific T cell stimulation.

But what are they? Why choose PepMix™ over whole proteins? What sort of pool formats are there? What antigens do they cover? What sort of quality grades do we offer? How soluble are they? What do you need to know when customizing your own peptide pool?

This fall, we’re unlocking all the secrets of PepMix™ peptide pools in a special series of newsletters that will answer all your burning questions — and more!

What’s Coming Up?

We’ve got a lineup of exciting topics that will guide you through everything you need to know about PepMix™:

What exactly is PepMix™? Discover why they’re the ultimate tool for immune monitoring and beyond. From overlapping peptides to diverse formats, we’ll break down how these pools are designed for maximum efficacy.

Immune Monitoring

Neo-antigens & Personalized

Medicine Gene Therapy & Immunogenicity

Viral antigens

Cancer antigens

Other antigens

Positive and negative control pools

Customization at your fingertips: What if you do not find your PepMix™ of interest in our catalog? Customize your PepMix™ Peptide Pool! What do you need to consider? Do you require additional modifications?

We also provide ELISpot services! Take your research further with our ELISpot services. Handling advice: Working with peptides requires a certain amount of knowledge. We will provide you with our user guidelines for adequate handling and reliable assay read-outs.

And that is not all! Look forward to a potential surprise for all our customers!

Don’t miss out & Stay tuned!

To make sure you do not miss any updates, be on the look-out for the upcoming newsletters! Don’t forget to follow us on Youtube, Linkedin and Facebook!

Connected reading

Helpful context for this guide

Source-derived material selected through this article’s indexed topics.

Research context

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JPT's Peptide Pools for Infectious Diseases Research

PepMix™ Peptide Pools are crucial tools for studying immune responses to infectious diseases, including viral and bacterial infections. JPT’s PepMix™ products enable researchers to assess T cell reactivity, improve vaccine formulations, and explore immune protection mechanisms. Below, you will find key studies referencing JPT’s PepMix™ Peptide Pools in various research.

Source: jpt.com ↗

TCR-T Research with PepMix™ Peptide Pools for Key Cancer Targets

Published on 15/05/2025 T-cell receptor-engineered T-cell therapy (TCR-T) is rapidly gaining momentum in the field of cancer immunotherapy. Unlike CAR-T cells, which recognize surface antigens, TCR-T cells can detect and eliminate cancer cells based on intracellular tumor-associated antigens presented by MHC molecules, offering broader therapeutic opportunities across solid and hematologic malignancies. A recent comprehensive review (Zhang et al., 2023) highlights the 16 major cancer targets currently pursued in TCR-T clinical trials. JPT is proud to support researchers in this exciting field by offering PepMix™ Peptide Pools for 15 of these 16 critical targets — and a partial PepMix™ for the remaining one (EGFRvIII). Main TCR-T Targets Available as PepMix™ Peptide Pools: AFP (Alpha-fetoprotein) EGFRvIII (partially available as ErbB2-ICD and ErbB2-ECD) EBV (Epstein-Barr Virus) HA-1 HBV (Hepatitis B Virus) HPV (Human Papillomavirus) KK-LC-1 KRAS (mutated) MAGE-A1 MAGE-A3 MAGE-A4 MAGE-A10 MART-1 Mesothelin NY-ESO-1 WT1 Check all our tumor-associated antigen peptide pools and our more than 1500 individual epitope peptides. Why Choose PepMix™ Peptide Pools for TCR-T Research? Comprehensive Antigen Coverage: Each PepMix™ consists of overlapping peptides that span the full protein sequence, ensuring maximal coverage of known and novel epitopes — ideal for discovery, monitoring, and functional assays. High Quality and Batch Consistency: Our optimized synthesis and stringent QC processes guarantee high purity, reproducibility, and biological performance you can trust for clinical research. Experience with Difficult Sequences: Hydrophobic and aggregation-prone sequences? No problem. JPT’s proven expertise ensures even challenging targets are fully represented in your peptide pool. Immediate Availability: More than 450 different catalog PepMix™ peptide pools are available from stock — enabling faster project initiation and smoother workflows. In case we do not have you antigen, contact us! We can provide it to you as a custom PepMix™ Peptide Pool. Accelerate Your TCR-T Research PepMix™ Peptide Pools are widely used for: Screening TCRs for target specificity Monitoring T-cell responses post-infusion Identifying immunodominant epitopes Supporting preclinical and translational studies Advance your TCR-T programs with reliable tools trusted by researchers worldwide and learn more about The World of PepMix™ or contact us!

Source: jpt.com ↗
Practical and safety references

These excerpts are educational, not personalised medical instructions.

Potential benefits

Benefits of Custom PepMix™ Peptide Pools

Overlapping peptide pools are preferable to whole proteins for antigen-specific T-cell stimulation because: Simultaneous detection of CD4+ and CD8+ responses in a single sample Equivalent or better stimulation T-cell responses compared to whole protein antigens Antigen spanning peptide pools stimulate T-cells in an HLA independent manner All epitopes covered, epitope spreading addressed Purified peptide pools eliminate risk of toxic inhibition of T-cell responses No false positive T-cell stimulation caused by deletion peptides Superior control compared to phytohemagglutinin (PHA) and concanavalin A (ConA) Improved responses in stored blood and PBM cells compared to whole proteins High-batch-to-batch reproducibility Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation Prolonged shelf stability when stored freeze dried

Source: jpt.com ↗
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Peptide Therapy Guide Editorial Team

Editorial team for Peptide Therapy Guide.

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