Understand the source comparison
TB-4 Research Returning Researcher FAQ: Model-Specific Comparison
Scratch assay (in vitro) 10–100 ng/mL in culture medium 7–14 days at 4°C in medium Refrigerated stock at 1 mg/mL, dilute fresh Cell line passage number. Higher passages show reduced TB-4 responsiveness Transwell migration 50–200 ng/mL in serum-free medium 48 h
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This page preserves a source comparison for education. It does not add a rating, recommendation or clinical judgment.
- Scratch assay (in vitro)
- 10–100 ng/mL in culture medium
- 7–14 days at 4°C in medium
- Refrigerated stock at 1 mg/mL, dilute fresh
- Cell line passage number. Higher passages show reduced TB-4 responsiveness
- Transwell migration
- 50–200 ng/mL in serum-free medium
- 48 hours at 4°C in medium
- Single-use aliquots at working concentration
- Serum lot variability. New FBS batches change baseline migration rates
- Cardiac injury model (rodent)
- 5–10 mg/kg subcutaneous or IP
- 4 weeks at 4°C (reconstituted)
- Multi-dose vials, refrigerated
- Animal supplier and diet changes affect baseline infarct size
- Wound healing (topical)
- 0.1–1.0 mg/mL in sterile saline or vehicle
- 14 days at 4°C
- Sterile single-use syringes
- Wound model technique consistency. New personnel require retraining
- Angiogenesis assay (tube formation)
- 100–500 ng/mL in reduced-growth-factor matrix
- 24 hours at 4°C in matrix
- Thaw matrix aliquot per experiment
- Matrix lot number. Basement membrane extract composition varies