Guide library
Plain-language education with dates, authors and original source links where available.
Table 1 Selection of cell-penetrating peptides (Reference: https://doi.org/10.1007/978-981-13-8747-0, Ülo Langel, CPP, Cell-Penetrating Peptides, 2019) Name Sequence 2 DSLKSYWYLQKFSWR (Kondo et al. 2012) 18A D…
Read guide →Alzheimer’s disease as we know it today is a horrible and currently incurable neurodegenerative disorder characterized by neuronal loss, memory impairment, and cognitive decline. Ongoing research is always loo…
Read guide →Fluorescent peptide labelling with Cy5, a cyanine dye, has become an indispensable technique in biomedical research, enabling the precise visualization and tracking of peptides in complex biological systems. T…
Read guide →How long do peptides last in the fridge? How should peptides be stored? For long-term storage, the peptide should be kept in solid form in the deep freezer at < -15 °C. If stored at room temperature some pepti…
Read guide →Fluorescent labelling is a cornerstone technique for visualizing and quantifying biomolecular interactions, with 7-Methoxycoumarin-4-acetic acid (MCA) standing out as a particularly versatile fluorophore for p…
Read guide →Fluorescent labeling with Cy5.5 has become a cornerstone in biomedical research, enabling the visualization and tracking of biological molecules in complex environments. This article delves into the specifics …
Read guide →Fluorescent labeling is a powerful technique used in cell biology and microscopy to visualize specific molecules within cells. Among the various fluorophores available, Texas Red stands out as a bright red-flu…
Read guide →Fluorescent labeling with FAM (carboxyfluorescein) has become a foundational technique for visualizing and tracking peptides in a wide range of biological systems. This bright, green fluorophore is prized for …
Read guide →Fluorescent peptides play a crucial role in biological research, enabling the visualization and tracking of molecules and processes. Among the various fluorescent dyes available, Alexa Fluor 647 stands out due…
Read guide →Fluorescence Resonance Energy Transfer (FRET) technology has revolutionized molecular detection by enabling real-time monitoring of biomolecular interactions and enzymatic activity. Among the most effective FR…
Read guide →How can I improve protein purity without reducing yield? I encountered a problem that is quite common when trying to purify proteins: I lose significantly more protein when trying to increase purity. I am curr…
Read guide →The FLAG peptide, also known as DYKDDDDK, is a short, hydrophilic, and highly charged peptide sequence. It is extensively utilized in the field of molecular biology for the detection and purification of protei…
Read guide →Biochemists are excited by the possibilities presented by peptides and proteins as pharmaceuticals because they so often mimic exactly the behavior of a natural ligand – the substance that interacts with the r…
Read guide →Epitope mapping identifies antigen regions that serve as binding sites for antibodies. The overlapping linear peptides derived from the primary sequence of the antigen are frequently used for epitope screening…
Read guide →As methods of medicine advance, targeted drug delivery becomes a more appealing and achievable option over its non-selective counterpart. It can focus solely on increasing therapeutic concentration in the targ…
Read guide →The process of linking a peptide to a carrier protein is a foundational technique in vaccine development and antibody production, enhancing the overall efficacy and response of the immune system to the antigen…
Read guide →A correct peptide design is crucial for generating working antibodies because it directly impacts the success and specificity of the antibody production process. Here are several reasons why peptide design pla…
Read guide →Western Blot: Technique, Theory, and Trouble Shooting # Western blotting is an important technique used in cell and molecular biology. Researchers can identify specific proteins from a complex mixture of prote…
Read guide →Protein-protein interactions (PPIs) are fundamental to virtually all biological processes, ranging from cellular signaling and structural organization to enzymatic activity and immunological recognition. Modul…
Read guide →Synthesis of multiple antigenic peptides: strategies and limitations Dendrimeric platforms such as MAPs can be synthesized by a solid phase method or by conjugation. Dendrimeric platforms such as MAPs can be s…
Read guide →