Top ELISpot Assay Services | JPT Peptide Technologies

ELISpot Service

Expert ELISpot Method Development & Troubleshooting Service!

Why Choose JPT for ELISpot Services?

Recognized Expertise: Our work is featured in the Handbook of ELISPOT (Schnatbaum et al., 2024), demonstrating our leadership in antigen-specific stimulation.

Proven Performance: In 2023, we achieved the 2nd best score in a global T-cell ELISpot proficiency panel accuracy test among 35 labs. Since 2017, we have been part of this international multiconsortia proficiency panel at the initiative of CIC and CIMT, and are currently still sponsoring tests with our unique CEFX Ultra SuperStim Pool. Its goal is to help researchers and clinicians worldwide evaluate and benchmark their performance in immune monitoring with T-cell ELISpot assays.

Optimized Protocols: We have developed and implemented harmonized, standardized protocols for reproducible and high-quality data.

Global Community Involvement: As part of the ELISpot Resource Group, we actively contribute to international workshops and knowledge exchange. Contact us if you are interested in hosting a workshop at your facility!

Take Advantage of our Comprehensive ELISpot Services

In Silico Peptide Design: Computationally optimized peptides to avoid synthesis and application challenges.

Custom Peptide Specifications: Choose from a range of peptide formats, purities, and modifications, including phosphorylation, acetylation, and more, to meet your unique requirements.

Experimental Design Consultation: Work with our experts to ensure meaningful results.

Standardized Experiment Execution: All assays follow harmonized guidelines and validated SOPs. Lab performance is continuously monitored annually by an independent proficience panel.

Professional Data Analysis & Reporting: Receive comprehensive analysis with expert interpretation.

Applications of JPT’s T-Cell ELISpot Service

ELISpot Method Development – Develop a robust protocol for your application.

ELISpot Troubleshooting – Analyze and resolve your ELISpot problems.

Epitope Discovery & Mapping – Identify key antigenic regions.

Immunomonitoring – Screen for antigen-specific immune responses.

Immunogenicity Testing – Detect unwanted immune responses to biologics

Our Comprehensive ELISpot Assay Service

At JPT, we offer a full spectrum of ELISpot Assay services, tailored to meet your specific research needs:

In Silico Peptide Design: Our expert team uses advanced computational tools to design peptides that avoid synthesis and application difficulties, ensuring optimal performance in your assays.

Customized Peptide Specifications: Choose from a range of peptide formats, purities, and modifications, including phospho, biotin, dyes, and more, to suit your unique requirements.

Experiment Design Consultation: Collaborate with our experienced scientists to design experiments that yield the most meaningful and actionable results.

Standardized Experimental Execution: All experiments are performed according to harmonized guidelines and validated SOPs, ensuring consistent, high-quality results.

Professional Data Analysis and Reporting: Receive comprehensive data analysis and reporting, complete with expert interpretation and discussion to help you understand and leverage your findings.

Ready to Elevate Your Research?

Partner with JPT for reliable, high-quality ELISpot assay solutions. Contact us today to discuss your project and find out how our expertise can advance your research!

Contact us!

What is ELISpot and Why is it Important?

ELISpot (Enzyme-Linked ImmunoSpot) is a highly sensitive technique used to detect and quantify cytokine-producing cells. This method is crucial for a wide range of applications in immunology, allowing researchers to measure the frequency of immune cells that produce a specific cytokine in response to a stimulus. ELISpot Assays are invaluable for advancing research in vaccine development, epitope mapping, and immunomonitoring.

Each individual peptide is purified and quality controlled

CoA and HPLC-MS data available for each individual batch

Capping to avoid false positive T-cell responses by deletion peptides

ADCF policy (animal derived component free)

Antigen Peptides

Individual immune dominant epitope peptides for different viruses, bacteria and other species for antigen-specific immune response profiling.

Each peptide is purified to > 90% (HPLC/MS)

Delivery within 2-5 days

HPLC-MS analyses included to confirm the identity and quality

More than 350 Antigen Peptides in stock from more than 40 different species!

Applications

Applications for ELISpot Service

Classic T-cell ELISpot using PBMC for cytokine quantification (e.g. IFN gamma quantification, interleukin quantification)

Dual FluoroSpot for simultaneous analysis of two different cytokines

B-cell ELISpot for enumeration of antibody secreting cells

Application Notes

"Strategy for Identification of CD8 T-cell Epitopes in a Viral Protein" by Holtappels Full Text

"CEFX/EFX ULTRA SuperStim Pools: Superior positive controls for T cell assays with broad MHC-allele and antigen coverage" by Eckey et al. Full Text

"A Fast and Low Cost Process for Neo-Epitope Based Immune Monitoring" by Derhovanessian et al . Full Text

"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy" by Lam et al. Full Text

"Cytomegalovirus Protein Spanning PepMix™ Peptide Pools to Discover Changes in T cell immunity in the Aging Population" by Kern Full Text

"T cell Therapy for Viral Infections After Hematopoietic Stem Cell Transplant" by Keirnan et al. Full Text

"Qualification and Use of Peptide Libraries for Clinical Trial Immunomonitoring" by Cox & Hayes Full Text

Benefits

Benefits of ELISpot Service

We offer additional techniques such as ELISA for further analysis

Reliable and robust workflows

Strict quality control

Competitive pricing

References

References for ELISpot Service

Read References with PepMix™ Peptide Pools

Read References with PepTrack™ Peptide Libraries

An Overview of Peptides and Peptide Pools for Antigen-Specific Stimulation in T-Cell Assays. In: Handbook of ELISPOT Schnatbaum, K. et al. (2024)

Elispot service

Elispot service

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