Educational guide
Tagged Protein Purification MagBeads
Tagged Protein Purification MagBeads Catalog Products » Protein Isolation and Purification » Magnetic Beads » Tagged Protein Purification MagBeads Overview 6xHistidine and Glutathione S-transferase (GST) are commonly used protein/peptide tags added to the end
This guide cannot diagnose a condition or recommend a personal treatment plan. Discuss medical questions with a qualified professional.
Tagged Protein Purification MagBeads
Catalog Products » Protein Isolation and Purification » Magnetic Beads » Tagged Protein Purification MagBeads
Overview
6xHistidine and Glutathione S-transferase (GST) are commonly used protein/peptide tags added to the end of a recombinant protein to aid in their purification or detection. GenScript Ni-Charged MagBeads and Glutathione MagBeads provide simple, rapid and reliable methods for micro-scale purification of His- and GST-tagged fusion proteins from crude cell lysate prepared from bacteria, yeast or mammalian cell culture.
The Ni-Charged MagBeads are pre-charged with nickel and ready to use for micro-scale purification of 6xHis-tagged proteins under native or denaturing conditions. The Glutathione MagBeads are immobilized with Glutathione mainly through its central carbon on the beads surface for efficient GST binding. Read More »
All the protocols are easily performed by applying the magnetic separation technology. They do not require the use of columns or centrifugation and all the steps take place in a single tube. The MagBeads can be used in very small volumes to acquire high concentrated target proteins. All the advantages make Ni-Charged MagBeads and Glutathione MagBeads the ideal tools for micro-scale purification of His- and GST- tagged fusion protein.
Alkaline-tolerant Resin
Tagged Protein Purification Resins
Protein A, G and L Resins
DYKDDDDK Tagged Protein Purification and Detection
Antibody purification MagBeads
Tagged Protein Purification MagBeads
Magnetic Racks
AmMag™ Magnetic Bead
AmMag™ Quatro ProAb 1300
Endotoxin Detection
ToxinSensor Single Test Kit
Endotoxin Removal
Product List
L00295
Ni-charged MagBeads
$101.00 8 ml (2 ml Settled Beads)
L00776
AmMag™ Ni Magnetic beads
$510.00 4 ml (1 ml Settled Beads) 40 ml (10 ml Settled Beads) 400 ml (100 ml Settled Beads)
L00835
MonoRab™ Anti-DYKDDDDK Magnetic Beads
$382.00 2 ml (1 ml Settled Beads) 10 ml (5 ml Settled Beads) 50 ml (25 ml Settled Beads)
L00895
Glutathione MagBeads
$135.00 2 ml (0.5 ml Settled Beads) 10 ml (2.5 ml Settled Beads)
L00936
Streptavidin MagBeads
$165.00 2 ml (0.5 ml Settled Beads)
Experimental Data and Citations Database
Lane 1, Bacterial lysate expressing GST Tag.Lane 2, Purified GST Tag (26 kDa) using Glutathione MagBeads.Lane 3, Bacterial lysate expressing His-tagged protein.Lane 4, Purified His-tagged protein (27 kDa) using Ni-charged MagBeads.
Fig.2. Purification of GST Tag and His-tagged Protein using GenScript Micro-scale Tagged Protein Purification MagBeads. Lane M, Protein Molecular Weight Standard.
Related Products
Protein A, G and A/G MagBeads
immunoprecipitation and micro-scale antibody purification.
immunoprecipitation and fast isolation.
Neuroscience Antibodies
Superior Antibodies for Neuroscience Research.
REQUEST A QUOTE
PHONE
1-877-436-7274 (Toll-Free) 1-732-885-9188
ONLINE FORM
Online Quote Submission
FAX
1-732-210-0262