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Solved Example: Net charge of a peptide NH, pka = 8 What is
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Question: Example: Net charge of a peptide NH, pka = 8 What is net charge on this peptide at pH 5, 7 and 12? What is the pl??? Ala CH-CH3 Solution: NH pKg = 4.07 CH-CH2-CH2-C00 o=4 VH SH 1. arrange the pka in ascending order • 3.5, 4.07, 8, 10.54 2. select the pH values below each pka 3. Check the net charge on the peptide 4. Select the 2 pka values (1 after and 1
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Example: Net charge of a peptide NH, pka = 8 What is net charge on this peptide at pH 5, 7 and 12? What is the pl??? Ala CH-CH3 Solution: NH pKg = 4.07 CH-CH2-CH2-C00 o=4 VH SH 1. arrange the pka in ascending order • 3.5, 4.07, 8, 10.54 2. select the pH values below each pka 3. Check the net charge on the peptide 4. Select the 2 pka values (1 after and 1 before) where the peptide has zero net Lys charge 5. Find the midpoint by taking average. NH pk = 10.54 CH-CH2-CH2-CH2-CH2-NH3 Coo pka = 3.5