Educational guide
Peptide Quantification for Precision Proteomics
Elevate Your Proteomics Assays: Peptide Quantification for Precision Proteomics Published on 07/09/2023 Absolute quantified peptides are used as internal references for protein quantification in mass spectrometry-based proteomics. Our proprietary peptide quant
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Elevate Your Proteomics Assays: Peptide Quantification for Precision Proteomics
Published on 07/09/2023
Absolute quantified peptides are used as internal references for protein quantification in mass spectrometry-based proteomics.
Our proprietary peptide quantitation method overcomes the limitations of traditional methods such as limited accuracy and high costs. We attach our QTag to each peptide, allowing robust and reproducible quantitation via HPLC-UV or HPLC-MS and UV. Digestion with trypsin in your sample releases the QTag and does not interfere with subsequent measurements.
Absolute Quantified Peptides - SpikeTides TQL
JPT’s QTag is attached to the peptide enabling peptide quantification via HPLC-UV, which is a robust, reproducible & accurate method (variation CV= 5%) with much lower cost than traditional methods. SpikeTides TQL have been used in numerous peer-reviewed publications.
Also, check our Catalog SpikeTides TQL
Histones
Metabolic Enzymes
Clinical Proteomics Peptides - SpikeTides TQL PLUS
Reference peptides for protein quantification in clinical proteomics are also quantified using our proprietary QTag before delivery. In addition, you yourself are able to perform an easy and reliable up-to-date peptide quantitation immediately before your experiment ensuring high accuracy for clinical applications.
Assay Standards & Controls
CAMCheck Kit Monitor the efficiency of carbamidomethylation during proteomic sample preparation
Retention Time Standardization Kit (PROCAL)Normalize retention times in proteomics experiments and optimize HPLC gradients
SpikeMix™ ABRF (cross-species standard) Use this pool of proteotypic peptides from conserved proteins as standard across human, mouse and rat
TrypCheck Kit Fluorescence Estimate efficiency & reproducibility of tryptic sample preparation
Did we spike your interest in our proteomics tools? Then check this further information on our website:
Research Areas: Proteomics The Proteome Tools Project The Human Proteome Peptide CatalogReferences with Proteomics Peptides
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