Peptide fluorescent labeling

Peptide fluorescent labeling

Please click here to get a quote for Fluorescent Peptide Services now!

Fluorescent peptides are short amino acid chains that emit light when exposed to certain wavelengths of light. These peptides are often used as probes in fluorescence microscopy, which allows for the visualization and tracking of biological molecules and processes. Additionally, fluorescent peptides can be used to monitor protein-protein interactions and enzymatic activities in real-time. The design and synthesis of fluorescent peptides involve various techniques, such as solid-phase peptide synthesis, click chemistry, and bioconjugation.

LifeTein offers an extensive range of fluorescent labeling options, including FITC, FAM, TAMRA, Cyanine Dye Cy3, Cy3.5, Cy5, Cy5.5, Cy7, Cy7.5, EDANS/Dabcyl, MCA, AZDye, BODIPY FL or Alexa Fluor (Alexa488, Alexa532, Alexa546, Alexa594, Alexa633, Alexa647), ATTO Dyes (Atto465, Atto488, Atto495, Atto550, Atto647), DyLight (DyLight 488, DyLight 550). Different fluorescent dyes cover a broad spectral range and offer various excitation and emission wavelengths to facilitate the detection of peptides using different fluorescence detection techniques, such as flow cytometry, live-cell imaging microscopy, and microarrays.

Methoxycoumarin (MCA)

360

410

317

Succinimidyl ester

Alexa 488 dye

490

525

699.7

FITC

495

519

389

pH sensitive

Cyanine Cy2

492

510

Cyanine Cy3

550

570

Alexa 555 dye

555

580

534.5

Rhodamine B

590

Abberior Star635

635

655

911

STED and Confocal Imaging

Cyanine Cy5

650

670

Silicon-Rhodamine (SiR)

652

674

472.61

Multicolour Live Cell Imaging

Fluorescent dyes are the primary means of labeling biomolecules. The most common such dyes are FITC derivatives, which have a wide range of applications in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays.

FRET Assay

FITC/Cy/ATTO

FAQs

Case Studies

Fluorescence Resonance Energy Transfer (FRET) using peptides

Standard dye combinations used for FRET:

Useful FRET calculator that provides a listing of key FRET pair information: www.fpbase.org/fret/

FITC and Dabcyl:FAM/Lys(DABCYL)

FITC and TAMRA: FAM/TAMRA

Methoxy-coumarin-acetic-acid(MCA) and 2,4-Dinitrophenyl(DNP): MCA/Lys(Dnp).

Ortho-aminobenzoic acid (Abz) and 2,4-dinitrophenyl (DNP) or N-(2,4-dinitrophenyl)ethylenediamine (EDDnp): ABZ/Tyr (NO2), ABZ/EDDnp

DABCYL and Glu(EDANS)

Cy3, Cy3.5, Cy5, Cy5.5, Cy7, Cy7.5, Alexa fluor, ATTO dyes and other Near Infrared (NIR) Fluorescent Dyes are available

Reference:

Ge, X., Wekselblatt, J. B., Elmore, S., Wang, B., Wang, T., Dai, R., Zhang, T., Dave, H., Ghaderi, M., Anilkumar, A. R., Wang, B., Sirsi, S. R., Ahn, M., Shapiro, M. G., Oka, Y., Lois, C., & Qin, Z. In Vivo Cytosolic Delivery of Biomolecules into Neurons for Super-Resolution Imaging and Genome Modification. Advanced Science, 2501033. https://doi.org/10.1002/advs.202501033

...FITC-Tet1, Aleax 594-N1, and Atto 643-N1 (95% purity) were acquired from LifeTein (NJ)...

An Angiotensin-(1-7) Endopeptidase in the Kidney Cortex, Proximal Tubules and Human HK-2 Epithelial Cells that is Distinct from Insulin Degrading Enzyme, American Journal of Physiology - Renal Physiology, 7 January 2015Vol. no. , DOI: 10.1152/ajprenal.00609.2014

Fluorescent peptide Angiotensin 1-7 (Ang-(1-7)) with FRET pair Abz/[Tyr7(NO2)] was synthesized by LifeTein. This FRET peptide was used to study the Ang-(1-7) endopeptidase in the renal renin angiotensin system. It was found that the endopeptidase is expressed within the renal proximal tubule. It may regulate Ang-(1-7) tone.

... 100 µM Abz-Ang-(1-7)- [Tyr7(NO2)], an internally quenched fluorescent peptide (synthesized by LifeTein, South Plainfield, NJ, USA) for 20 hours or 0.5 nM 125I-(Ang-1-7) for 60 minutes at 37°C ...

Cell Penetration Monitoring

Fluorescent dyes are used to monitor the cell penetration of peptides and conjugated to various antibodies, proteins, and peptides for cellular labeling and detection. LifeTein offers to conjugate peptides to fluorescent cell-penetrating peptides (CPPs) of your choice, including R8, TAT, Maurocalcine, and penetratin, to detect their cell penetration using microscopy.

ATTO Dyes

Atto dyes can be used to replace regular fluorescent dyes. Atto dyes have enhanced photostability and ozone resistance, long signal lifetimes, and reduced background for higher sensitivity. They are ideal for multiplex techniques using visible and near-IR emission wavelengths. For example, Atto 488 is a superior alternative to FITC and Alexa Fluor 488, producing conjugates with more photostability and brighter fluorescence. Atto 550 is an alternative to rhodamine dyes, Cy3, and Alexa Fluor 550, offering more intense brightness and increased photostability.

Alexa Fluor 488

Atto 488

FAM

JOE

Atto 520

TET

Alexa Fluor 532

Atto 532

HEX™

Atto 532,Atto Rho6G

TAMRA

Atto 550

Cy3

Cy3.5

Atto 565

ROX

Atto 565,Atto Rho11

Alexa Fluor 594

Atto 590,Atto 594

Texas Red

Atto 590

Alexa Fluor 633

Atto 633,Atto Rho14

Cy5

Atto 647,Atto 647N,Atto 655

Alexa Fluor 647

Cy5.5

Atto 680,Atto 700

Frequently Asked Questions: Peptide Synthesis

Please click here to see more FAQs

Case Study

The client requested a very hydrophobic peptide 68 amino acid in length (85% purity) with FITC modification at the N-terminus. The peptide was successfully synthesized in 4 weeks.

HPLC Results:

MS Results:

Method 1: Obtain a quote. Please use the online quotation form:

Method 2: Please complete the Quotation Form for the peptide you would like to purchase and either fax it to 1-888-791-1618 or email it to [email protected]. We will send you a quote within 24 hours.

Method 1: Place your order online if you already have a quote number:

Method 2: Please sign the quote form we issue to you, and either fax it to 1-888-791-1618 or email it with your Purchase Order, if any, to [email protected]. We will confirm your order within 24 hours.

We use essential cookies to make our site work. With your consent, we may also use non-essential cookies to improve user experience and analyze website traffic. You can accept all cookies or continue with essential cookies only. See our Cookie Policy.