Educational guide
PE-22-28 Dosage Protocol | PeptideDosages.com
PE-22-28 (10 mg Vial) Dosage Protocol PE-22-28 Dosage Chart PE-22-28 is dosed at 50 mcg–200 mcg daily via subcutaneous injection in educational protocols. A 10 mg vial reconstituted with bacteriostatic water yields about 3.33 mg/mL. This information is for res
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PE-22-28 (10 mg Vial) Dosage Protocol
PE-22-28 Dosage Chart
PE-22-28 is dosed at 50 mcg–200 mcg daily via subcutaneous injection in educational protocols. A 10 mg vial reconstituted with bacteriostatic water yields about 3.33 mg/mL. This information is for research and educational use only.
Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.
Typical daily range: 50–200 µg once daily (gradual titration over 8–16 weeks).
Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 µg on a U-100 insulin syringe.
Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); protect from light.
PE-22-28 is a synthetic heptapeptide (sequence: GVSWGLR) derived from the sortilin propeptide, engineered as a potent and selective antagonist of TREK-1 potassium channels[4]. Preclinical studies demonstrate rapid antidepressant-like effects, enhanced neurogenesis, and neuroprotection with a favorable safety profile showing no cardiac or metabolic side effects[2][3]. This educational protocol presents a once-daily subcutaneous approach with gradual titration.
Research context: For evidence on mechanisms, human and preclinical research, limitations, and safety, read PE-22-28 Peptide: Benefits, Uses, Side Effects, Dosage, and Research.
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
Weeks 1–2
50 µg
1.5 units (0.015 mL)
Weeks 3–4
100 µg
3 units (0.03 mL)
Weeks 5–8
Weeks 9–12 (Optional)
150 µg
4.5 units (0.045 mL)
Weeks 13–16 (Optional)
200 µg
6 units (0.06 mL)
Frequency: Inject once daily subcutaneously. This schedule uses a practical 3.0 mL dilution for accurate measurement. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability. Many researchers find 100–150 µg sufficient given PE-22-28’s high potency[4].
Reconstitution Steps
Draw 3.0 mL bacteriostatic water with a sterile syringe.
Inject slowly down the vial wall; avoid foaming.
Gently swirl/roll until dissolved (do not shake).
Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Supplies Needed
Plan based on an 8–16 week daily protocol with gradual titration. Replace vials every 4 weeks to maintain potency.
Peptide Vials (PE-22-28, 10 mg each):
8 weeks ≈ 2 vials
12 weeks ≈ 3 vials
16 weeks ≈ 4 vials
Insulin Syringes (U-100):
Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes
Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
8 weeks (2 vials): 6 mL → 1 × 10 mL bottle
12 weeks (3 vials): 9 mL → 1 × 10 mL bottle
16 weeks (4 vials): 12 mL → 2 × 10 mL bottles
Alcohol Swabs: One for the vial stopper + one for the injection site each day.
Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100-count boxes
12 weeks: 168 swabs → recommend 2 × 100-count boxes
16 weeks: 224 swabs → recommend 3 × 100-count boxes