Mammalian Protein Expression Service (U.S.-Based)

Mammalian Protein Expression Service (U.S.-Based)

LifeTein provides recombinant protein expression in mammalian systems, including HEK293 and CHO expression platforms, for projects that require correct folding, mammalian post-translational modification, and native biological activity.

This service is used for recombinant proteins, secreted proteins, and antibody expression projects where the target depends on eukaryotic expression for correct performance in downstream functional or structural studies.

Service Overview

Expression systems

HEK293 and CHO mammalian expression platforms

Typical use

Proteins requiring native folding, secretion, post-translational modification, or antibody expression

Workflow

Gene synthesis, plasmid preparation, transient expression, purification, and recombinant protein delivery

Expression modes

Transient expression and project-appropriate production workflows

Location

New Jersey, USA

When to Use Mammalian Expression

The target requires post-translational modifications such as glycosylation

Correct folding and native biological activity are critical

The protein is secreted or requires mammalian processing

Antibody expression or recombinant antibody production is needed

Functional studies depend on a native mammalian conformation

U.S.-Based Mammalian Expression

Mammalian protein expression work is carried out in LifeTein’s New Jersey, USA facility, providing a clear domestic workflow for projects where location matters.

Integrated Gene-to-Protein Workflow

LifeTein supports codon optimization, gene synthesis, plasmid preparation, mammalian expression, purification, and delivery of purified recombinant protein.

HEK293 and CHO Platforms

Suspension culture expression in HEK293 or CHO systems can be selected according to the target protein and project needs.

Antibody Expression Support

The service also supports recombinant monoclonal antibody expression workflows, including projects starting from antibody sequence or hybridoma-derived variable regions.

Mammalian expression is particularly important when experimental performance depends on correct folding, native epitope presentation, secretion behavior, or appropriate post-translational modification. In these cases, bacterial expression may not be sufficient even if it is faster or less expensive.

What We Support

Codon optimization and synthetic gene preparation

Plasmid preparation and cloning into mammalian expression vectors

Transient protein expression in HEK293 or CHO systems

Purification of recombinant proteins and antibody products

Sequence-based antibody expression projects

Flexible scale from analytical production to larger research batches

Antibody and Sequence-Based Expression Projects

LifeTein also supports hybridoma sequencing or antibody-sequence-based projects for recombinant monoclonal antibody expression. Variable-region sequences can be incorporated into mammalian expression vectors for transient or other project-appropriate antibody production workflows.

Need a faster bacterial option?

For rapid and cost-efficient protein production when mammalian post-translational modification is not required, see our Bacterial Protein Expression Service.

Frequently Asked Questions

How long does a mammalian protein expression project usually take?

Project timing depends on whether gene synthesis, plasmid preparation, expression optimization, purification, and characterization are included. Mammalian workflows are typically chosen when the biological quality of the protein is more important than maximum speed.

Why choose mammalian expression instead of bacterial expression?

Mammalian systems are used when the target requires correct post-translational modification, secretion, or native folding for the intended assay or biological function.

What materials can I send to start a project?

You can start with a protein sequence, gene sequence, plasmid, antibody sequence, or hybridoma-related material, depending on the scope of the project.

Quotation

Email your protein expression inquiry to [email protected]. We will send you a formal quote within 24 hours.

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