IVT RNA Synthesis Services

IVT RNA Synthesis Services

From Design to Delivery: mRNA, saRNA, circRNA & LNP Formulation

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One Stop Solutions for IVT RNA

GenScript’s fully integrated RNA platform spans mRNA, circular RNA, and self-amplifying RNA - seamlessly connected to LNP delivery systems and powered by patented GenScript Cap™ Technology for enhanced stability, efficiency, and targeting.

Optimized through our proprietary production platform, every RNA we deliver meets uncompromising quality standards - supporting your journey from early discovery through preclinical, IND, and early-phase clinical studies.

Rush mRNA Synthesis New!

GMP-like mRNA Manufacturing Services

Catalog IVT mRNA, circRNA, saRNA, LNP products

mRNA QC Analysis

GenScript Cap Technology

Circular RNA Synthesis

Self-Amplifying RNA

LNP Formulation

Targeted LNP Formulation

ReadyEdit LNP Formulation

Why GenScript

Accelerate Discovery

Industry-leading 2 weeks turnaround time from Gene-to-mRNA for global customers

Integrated capabilities from sequence design to RNA synthesis and LNP formulation in one solution

Delivered >10000 quality mRNA orders globally with 93% on-time delivery

Drive Innovation

Cutting-edge mRNA, circular RNA, and self-amplifying RNA technologies, seamlessly integrated with LNP delivery systems

GenScript Cap mRNA with high capping efficiency and UTR options

Cap2 mRNA for better expression at stressed condition

Circular RNA with enhanced circularization efficiency and high purity

saRNA with reduced immunogenicity and high expression

Cultivate Collaboration

Proprietary & patented GenScript Cap™ technologies open to licensing

Patented technologies covers:

Co-transcriptional capping Method

Selection of UTRs for tissue specific targeting

Optimized PolyA vector

New Cap Analogue 2’-O-Ethyl-capped RNA Enhance Half Life In Vivo

The 2′-O-Ethyl-capped mRNA delivers the highest and most durable expression profile among all tested cap analogs, making it an optimal choice for in vivo applications requiring sustained protein output.

One-Stop Solution for ALL Your mRNA Needs

GenScript’s solution to IVT mRNA manufacturing streamlines your workflow from gene synthesis to mRNA production. Our IVT mRNAs are optimized with our proprietary production platform, ensuring the quality and expression efficiency of your mRNA.

Codon/UTR Optimization

Codon optimization for protein expression

Codon optimization with >200 factors screened (patent)

Screened and validated UTR sequences

Gene Synthesis

100 bp – 200 kb gene inserts

TAT as fast as 7 BD to 14 BD

Gene synthesis with Poly(A)-built-in vectors

Cloning/Plasmid Synthesis

Ready-to-use linearized plasmid DNA for IVT

Vectors encoded stable Poly(A) tracts with variance of only +/- 5 nt

mRNA Synthesis

RUO to GMP-like grade

Advanced purification (Oligo dT, dsRNA removal) and variety of QC options

Lipid Nanoparticles Delivery

Generic LNP, Targeted LNP and ReadyEdit LNP (for gene editing)

Load 0.1 mg ~ Gram Level mRNA/circRNA/saRNA/siRNA/ASO/DNA

Load up to 20 kb mRNA

5' Cap Options

No Cap

(3'-NHAc-m7)G(5')pppA(2'O-Me)pG

(3’-OMe-m7)G(5')pppA(2’O-Etl)pG

2’-Oethyl Cap1

Cap2 (Co-Capped)

Cap0

Other (Customer provided Cap analogues)

Poly(A) Tail Options

100A Tail (recommended)

Custom tail ≤ 120A

No Tail

Modified NTP Options

N1-Me-Ψ

5moU

Ψ

5meC

Cy5-UTP or Thio-CTP

Unmodified

UTRs for tissue specific targeting

A549

HEK293T

Hela

Jurkat

HepG2

Popular off-the-shelf mRNA catalogs available now!

Try our catalog mRNA expressing reporter genes, Cas protein, or SB-100 transposase, all delivered in just 5 days!

Comparison of different IVT RNA payload formats

Linear mRNA

Self-Amplifying RNA

Circular RNA

Structure

Linear; Open 5’ and 3’ end mRNA exonuclease degradation

Close loop; No free 5’ 3’ end Eliminating mRNA exonuclease degradation

Ribosome recruiting

5’ Cap: Initiates translation & stabilizes mRNA

Internal Ribosome Entry Site (IRES) : Initiates translation

ORF

Codon optimization for translation yield

Modified nucleosides, eg. M1Ψ

Insert < 20 kb

Modified nucleosides, eg. M5C

Insert < 6 kb

Insert < 4 kb

UTR

Regulatory elements in the 5’ and 3’-UTR stabilize mRNA and increase translation initiation

Replicon for further self-replication of the mRNA

Scarless circRNA options for coding and non-coding applications

PolyA tail

Stabilizes RNA molecule & Prevents degradation

Not required

Protein Expression

+++ Strong

+++++ Strongest

++++ Stronger

Expression half-life

+++ Shorter

+++++ Longest

++++ Longer

Immunogenicity

++++ Low

++ High

+++ Medium

Dosage requirement

+++++ Lowest

Novelty

+++ Traditional

++++ Novel

+++++ Novel

Testimonials

Dr. Sean Whelan

Washington University School of Medicine

Contracting with Genscript for the production of mRNA-LNPs has advanced my laboratory's research into viral vaccines by allowing us to rapidly produce and test multiple mRNA vaccine constructs for several different viruses. We appreciate Genscript's easy ordering processing, and diverse options for mRNA format and LNP encapsulation strategy.

Dr. Mahdi Zeraati

University of Sydney

We have used their IVT mRNA synthesis, FLASH gene synthesis, and TurboCHO antibody production platforms across several projects, and each has consistently met our expectations. Notably, GenScript successfully synthesized a challenging, repeat-rich gene that other providers declined to undertake, demonstrating their technical capability and commitment to supporting complex research needs.

Chun-YuChen

Seattle Children's Hospital

I purchased mRNA from different companies and found that GenScript's mRNA showed higher expression (almost 2 fold) and much more cost effective!!

Dr. Sang-Moo Kang

Institute for Biomedical Sciences at Georgia State University

GenScript mRNA has helped us make a rapid progress in developing novel vaccine projects. GenScript's service is faithful and timely provided, saving me lots of time to focus on research. This is why I have been using GenScript services for over 20 years.

Zack Ho

R&D Manager at Lion TCR Pte Ltd

We have utilized GenScript's gene and mRNA synthesis service for our research projects, and the outcomes have been exceptional. The product quality, timely delivery, and technical support have consistently surpassed our expectations.

Resources

mRNA Case Study Report

Case studies showing high expression efficiency of GenScript’s mRNA for expressing SB transposase, GFP, Luciferase, eSpCas9, and more.

Circular RNA Case Study Report

Circular RNA introduction, GenScript offering details, and case studies showing increased stability, prolonged expression, and lower immunogenicity compared to linear IVT mRNA.

LNP Case Study Report

Case studies demonstrating encapsulation and delivery efficiency of GenScript’s new LNP formulations in cell models, and mouse models via both IM and IV.

Critical Consideration in Sourcing mRNA_Whitepaper

This whitepaper provides valuable insights into the essential factors that vaccine and therapy developers should consider when exploring mRNA solutions

Advancements in mRNA manufacturing_eBook

Get GenScript’s free application eBook on therapeutics development with mRNA manufacturing solutions

mRNA Therapy Modalities_Infographic

Learn about key structural features and functions of IVT mRNA and LNP, therapeutic modalities beyond prophylactic vaccines, mRNA candidates progressing through clinical trials.

mRNA Parts_Infographic

Learn about the main structural components of mRNA, the functional roles of each part, what constitutes high-quality IVT mRNA and more.

Circular RNA Beginner’s Guide_Infographic

Learn about key structural components of circRNA, the process by which circRNA is translated into protein, and more.

Self-Amplifying RNA Beginner’s Guide_Infographic

Learn about key structural components of saRNA, the processes by which saRNA replicates and is translated into protein, and more.

FAQs

We can make mRNA from 100bp to 20kb in length. For oligo shorter than 100bp, our oligo synthesis team can take it under chemical syntehsis.

For RUO grade mRNA, we use silica membrane or LiCl precipitation method for purification. It is suitable for in vitro and invivo proof of concept studies.

For preclinical grade, we use HPLC based Oligo dT purification for improving RNA purity. Preclinical grade can be considered for long mRNA constructs >5kb or when high RNA purity (less fragment RNA ) is required for the project.

We can provide cGMP mRNA through our sister company ProBio. Please contact our technical support team.

Yes. We guarantee specific amounts of mRNA be delivered.

We ship mRNA at concentration of 0.5 ~2mg/mL by default. We can adjust concentration to a specific value <4mg /mL per customer's request at extra cost.

We offer 3 options of buffer: 1mM sodium citrate pH 6.5, TE pH7, and RNase-Free H2O. 1mM sodium citrate is our default option for better mRNA stability.

mRNA should be stable for at least 1 year if stored properly at -80°C upon receival and avoiding repeated freezing and thawing cycles, however mRNA stability varies depending on the length of mRNA. We have tested mRNA with stable results up to 3 freeze/thaw cycles. We recommend storing at -80°C for long time storage and -20°C for short term storage.

For RUO grade mRNA, we use silica membrane or LiCl precipitation method for purification.

For preclinical grade, we use HPLC-based Oligo dT purification for improving RNA purity.

In addition, we offer optional dephosphorylation to further reduce triphosphate RNA species in the final product to reduce its immunogenicity, and optional dsRNA removal HPLC purification to provide higher quality mRNA if low dsRNA residue is required.

Cellulose based HPLC method.

For size-based purity, we run the mRNA sample on bioanalyzer, then calculate the ratio of the main peak (target size +-15%) over the total AUC.

We recommend customers dephosphorylation as reducing the triphosphate-RNA species in mRNA by dephosphorylation could reduce its immunogenicity, thus making the transfected cells healthier and can express the target protein better.

Modifications help stabilize mRNA and reduce its immunogenicity.

We can substitute uridine bases with either 5-moU, N1-me-Ψ, Ψ, 5’-iodourdine, or Cy5-UTP.

We can substitute cytidine bases with 5-me-C, 5’-iodocytidine, or thiol-CTP.

We can substitute customizable percentage substitutions upon request. These will be not site specific modifications.

Capping can stablize RNA from 5'end and recruit ribosome. Cap1 and Cap 2 have a higher impact on mRNA stability compared to Cap 0 regarding protecting the mRNA from exonuclease degradation. It is common to use Cap1 for mammalian cells and Cap 0 for plant cells. Cap2 is a novel solution provided by GenScript that may work better than Cap1 in mammailan cells especiailly in immune-stressed conditions.

Cap1 via co-transcriptional capping is our default recommendation. We can also offer Cap0 and Cap1 enzymatically for an additional cost, and Cap2 RNA through co-transcriptional capping technology.

GenScript's proprietary capping technology can achieve >97% capping efficiency.

Yes, we offer mRNA QC service including capping efficiency assay using enzyme digestion /LC-MS method. Please contact our technical support team for details.

We use linearized DNA plasmid as a template for manufacturing your mRNA. The gene can be designed by our team using GenScript’s proprietary vectors and tools or we can use one of your designs. Once the design has been agreed to, we handle the gene and mRNA manufacture and provide you with mRNA as part of the fee-for-service agreement.

Yes, it is possible, please contact our technical support team.

For making cap1 mRNA using co-transcriptional capping technology, either AGG or GGG following T7 promoter seq is required. For self-amplifying RNA usually ATG after T7 promoter.

Yes, we can do codon optimization. Please contact our technical support team.

Yes. we can subclone your ORF sequences into our proprietary vector which contains T7 promoter, UTR and 100A for mRNA production.

We use RNaseT1.

Yes, please refer to https://www.genscript.com/mrna-qc-services.html.

Approximately 0.5ug to 1 ug of mRNA is usually used for transfecting each well in 24 well plate.

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EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262