Educational guide
How to screen small peptide ligands for targeting receptors in cancer cells? | LifeTein Peptide Blog
Design a biotin-labeled peptide library: overlapping or Alanine scan The peptide library is coated on the avidin-coated 96 well plates. The Peptide library is used to evaluate the binding of each peptide to different cell lines using CyQUANT Cell Proliferation
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Design a biotin-labeled peptide library: overlapping or Alanine scan
The peptide library is coated on the avidin-coated 96 well plates.
The Peptide library is used to evaluate the binding of each peptide to different cell lines using CyQUANT Cell Proliferation Assays.
Flow cytometry: Determine the cellular uptake of selected FITC-labeled peptides.
Competitive assay: The cells are incubated with FITC-peptide with or without excess unlabeled peptide (100-fold)
Finetune the peptide sequence: structure analysis, cyclization, and D amino acid modification.
Find the promising receptor-specific cancer cell binding peptide that can be conjugated directly to a chemotherapeutic drug or to nanoparticles for targeted drug delivery to enhance the efficacy of chemotherapy for cancer treatment.
Reference:
https://www.nature.com/articles/s41598-019-38574-y