Educational guide
How to Reconstitute Hexarelin: Step-by-Step BAC Water Protocol & Concentration Calculator | Palmetto Peptides
Hexarelin Reconstitution Calculator: BAC Water Volumes & Concentration Tables for Lab Research DISCLAIMER: Hexarelin is a research chemical intended exclusively for qualified laboratory use in preclinical and in vitro research settings. It is not approved by t
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Hexarelin Reconstitution Calculator: BAC Water Volumes & Concentration Tables for Lab Research
DISCLAIMER: Hexarelin is a research chemical intended exclusively for qualified laboratory use in preclinical and in vitro research settings. It is not approved by the FDA for use in humans or animals. All concentration calculations described on this page are provided solely for laboratory research purposes. Nothing herein constitutes medical advice, dosing guidance, or instructions for self-administration. Always follow your institution's standard operating procedures and biosafety requirements. Palmetto Peptides supplies research-grade peptides for scientific investigation in compliance with all applicable law.
Last Updated: May 2026 | Reading Time: ~8 minutes | Author: Palmetto Peptides Research Team
Hexarelin is a potent synthetic hexapeptide GHRP that has attracted research interest beyond its GH-secretagogue activity — notably for its independently documented cardioprotective effects in animal models. Researchers working with Hexarelin for either GH axis studies or cardiovascular tissue research require the same foundational starting point: a precisely reconstituted research solution at a known concentration.
This page provides concentration lookup tables for Palmetto Peptides' 5mg Hexarelin vials, with pre-calculated BAC water volumes for every common working concentration used in preclinical research settings.
For the full step-by-step reconstitution procedure — equipment, aseptic technique, and troubleshooting — see our Research Peptide Reconstitution and Dosing Reference Guide. This page focuses on the Hexarelin concentration math.
Last Updated: May 27, 2026 | Reading Time: Approximately 5 minutes | Author: Palmetto Peptides Research Team
Quick Answer
Hexarelin: Research Background
Hexarelin (His-D-2-MeTrp-Ala-Trp-D-Phe-Lys-NH2) is a synthetic hexapeptide growth hormone-releasing peptide and potent agonist of the ghrelin receptor (GHSR1a). It stimulates growth hormone secretion from the pituitary gland in animal models, but unlike more selective GHRPs such as Ipamorelin, Hexarelin also activates additional receptors including CD36, which has made it a subject of interest in cardiovascular and cardioprotective research.
Preclinical research has examined Hexarelin in the context of GH secretagogue comparisons, cardiac tissue protection, and ischemia models. It is one of the few GHRPs with documented receptor activity outside the pure GHSR pathway, giving it a distinct research profile from compounds like Ipamorelin, GHRP-2, and GHRP-6.
Palmetto Peptides supplies Hexarelin as lyophilized powder in 5mg vials, with third-party HPLC purity verification (≥98%) and mass spectrometry identity confirmation.
View Hexarelin Research Peptide →
The Reconstitution Formula
Volume to add (mL) = Peptide mass (mg) ÷ Target concentration (mg/mL)
Use actual COA fill weight — not the labeled amount — for precise concentration control.
Hexarelin Concentration Calculator — 5mg Vial
0.5 mg/mL
10.0 mL
1.0 mg/mL
5.0 mL
2.0 mg/mL
2.5 mL
5.0 mg/mL
1.0 mL
Based on nominal fill weight of 5.0mg. Adjust using actual COA fill weight.
Adjusting for Actual COA Fill Weight
Example: COA fill weight = 4.91mg, target = 1.0 mg/mL Volume to add = 4.91 ÷ 1.0 = 4.91 mL BAC water
In comparative GHRP research designs (Hexarelin vs. Ipamorelin vs. GHRP-6), fill-weight-adjusted calculations for each compound in parallel arms ensures concentration-matched experimental conditions.
GHRP Comparison: Reconstitution Reference
Hexarelin is commonly studied alongside other GHRPs. The reconstitution math is identical for all — only mass, vial size, and target concentration differ:
Hexarelin
5mg
5 ÷ conc = mL BAC water
Less selective; CD36 agonism
Ipamorelin
5mg, 10mg
mass ÷ conc = mL BAC water
Highly selective for GH
Sermorelin
GHRH analog (different receptor)
CJC-1295
Solvent Selection
Bacteriostatic water (0.9% benzyl alcohol) is the recommended reconstitution solvent for Hexarelin research solutions stored between experimental uses. The benzyl alcohol preservative extends solution stability during refrigerated storage.
Sterile water for injection is appropriate only for immediate single-use protocols without storage requirements.
Palmetto Peptides supplies Bacteriostatic Water (BAC Water) 10mL vials for research reconstitution.
Aliquot Volume Reference
100 aliquots
200 aliquots
50 aliquots
25 aliquots
10 aliquots
20 aliquots
Storage Conditions
Lyophilized (unreconstituted)
-20°C, desiccated
Stable for 24+ months when properly stored
Reconstituted in BAC water
2–8°C, protected from light
Follow lab SOPs for peptide solution stability
Aliquoted samples
-20°C
Limit freeze-thaw cycles; thaw only what is needed
Frequently Asked Questions
How much BAC water for a 5mg Hexarelin vial at 1 mg/mL? Add 5.0 mL. This yields 5.0 mL of 1 mg/mL Hexarelin research solution.
How much BAC water for a 5mg Hexarelin vial at 2 mg/mL? Add 2.5 mL. Formula: 5 ÷ 2 = 2.5 mL.
Does Hexarelin dissolve easily in BAC water? Yes. Lyophilized Hexarelin is soluble in aqueous solvents. Gentle inversion or rolling for 30–60 seconds after adding BAC water is typically sufficient. No heating or extended mixing is required.
Is Hexarelin only available in 5mg vials? Palmetto Peptides currently supplies Hexarelin in 5mg vials. This vial size is sufficient for most research protocols. Researchers needing larger quantities can order multiple vials.
How does Hexarelin's cardioprotective research differ from its GH secretagogue research? In GH research, Hexarelin acts via the GHSR1a (ghrelin receptor) in the pituitary to stimulate GH release. In cardioprotective research, it acts via CD36 receptors in cardiac tissue — a receptor-specific effect that appears independent of GH secretion. Research designs studying the cardioprotective properties of Hexarelin often use models designed to isolate CD36 activation from GHSR-mediated effects.
How does Hexarelin compare to Ipamorelin in GH research? Hexarelin is a more potent GHRP but less selective — it stimulates cortisol and prolactin in addition to GH at research concentrations in animal models. Ipamorelin has significantly greater selectivity for GH release with minimal cortisol and prolactin stimulation. The choice between them depends on whether selectivity or potency is the research priority. See our Ipamorelin vs. GHRP-2 and GHRP-6 research comparison for a detailed comparison of GHRP selectivity profiles.
Source Your Hexarelin from Palmetto Peptides
Palmetto Peptides supplies Hexarelin with third-party HPLC purity verification (≥98%), mass spectrometry identity confirmation, and lot-specific COA documentation with actual fill weights.
Order Hexarelin Research Peptide →
Related research tools:
Ipamorelin Research Peptide — selective GHRP for comparative GH secretagogue research
CJC-1295 No DAC Research Peptide — GHRH analog frequently paired with GHRPs
Sermorelin Research Peptide — endogenous GHRH analog for comparative research
Bacteriostatic Water (BAC Water) — pharmaceutical-grade reconstitution solvent