Histone Code Peptide Microarray

Histone Code Peptide Microarrays

US$751.40

Excluding tax and shipping fees

In stock

Description

About Histone Code Peptide Microarrays

Extensive peptide library covering all known natural variants of histone sequences and modifications displayed on one microarray (> 3800 peptides).

Histone Code Peptide Microarrays - Specifications

Amount: 1 microarray (3 subarrays per slide)

Delivery Format: 1-sample peptide microarray

Application(s): Enzymology, Protein-protein-interaction

Condition(s)/Topic(s): Acetyltransferase, Cancer, Epigenetics, Kinase, Phosphatase

Standard Delivery Time: approx. 3 weeks

Are you interested in your custom Peptide Microarrays? Choose your sequences, and array format. We will assist you along the way. Custom PepStar Peptide Microarrays.

Histone Code Peptide MicroarrayAs histones are a major focus of epigenetics, JPT's computational scientists compiled this unique library of synthetic post-translationally modified and unmodified human histone peptides that is now available on a single peptide microarray or as individual biotinylated peptides. The peptide library consists of more than 1400 20mer peptides from Histone H1, H2A, H2B, H3 and H4. Peptides carry PTMs at reported sites (e.g. acetylation, methylation, phosphorylation, citrullination...) in various combinations representing all potential variants. This library is supplemented by 2444 peptides from an overlapping peptide scan through histone proteins carrying no or single modifications at reported sites.

Benefits of JPT's Histone Code Peptide Microarrays- Get hundreds of identical microarray copies- Small amounts of your precious samples are needed for incubation- Applicable to a variety of biological samples (e.g. serum, lysate, purified proteins, antibodies)- Low background on glass surface, read-out via fluorescence- Unprecedented resolution for mapping of protein-histone interactions due to coverage of natural sequence variants - Screen thousands of potential peptide substrates economically

References

References for Histone Code Peptide Microarrays

References:Read References with Histone Code Tools

Application Notes for Enzyme Substrate MicroarraysExploring the human acetylome using high-density peptide microarraysMasch et al., Application Note (2012) (full text)Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells Rao et al., Application Note (2016) (full text)Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex Wilczek et al., Application Note (2013) (full text)

Testimonial“JPT's new extremely high-density histone peptide array is a dramatic leap forward for analysis of the histone code hypothesis. The complete sequence coverage across core histones and histone variants as well as the presence of common and rare histone posttranslational modifications – alone and in combination – makes this an unparalleled tool for studying the “writers” and “readers” of the histone code. My laboratory has used these arrays to ask the sequence and PTM dependence of a histone writer enzyme and we have discovered an activity specifying code that will keep us busy for years to come.”David Shechter, PhD (Department of Biochemistry, Albert Einstein College of Medicine, YU, NY, USA)

Documentation

Documentation for Histone Code Peptide Microarrays

Protocol_HistoneCodeMA.pdf

Histone-Code-Peptide-Microarrays.pdf

Properties

Properties of Histone Code Peptide Microarrays

1 microarray (1" x 3")

Enzymology, Protein-protein-interaction

Enzyme Substrate Arrays

Acetyltransferase, Cancer, Epigenetics, Kinase, Phosphatase

1-sample peptide microarray, 21-sample peptide microarray

Human

Histones, Kinase

No

Further Information to Histone Code Peptide Microarrays

Values

20 mer peptides selected from natural histone protein variants