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HCG Dosage Protocol | PeptideDosages.com

HCG (5000 IU Vial) Dosage Protocol HCG Dosage Chart HCG is dosed at 500 IU weekly via subcutaneous injection in educational protocols. Reconstitute the 5000 IU vial with bacteriostatic water before use. This information is for research and educational use only

Written by Peptide Therapy Guide Editorial Team
For education only

This guide cannot diagnose a condition or recommend a personal treatment plan. Discuss medical questions with a qualified professional.

HCG (5000 IU Vial) Dosage Protocol

HCG Dosage Chart

HCG is dosed at 500 IU weekly via subcutaneous injection in educational protocols. Reconstitute the 5000 IU vial with bacteriostatic water before use. This information is for research and educational use only.

Reconstitute: Add 2.0 mL bacteriostatic water → 2,500 IU/mL concentration.

Typical dosing: 500 IU subcutaneous, 3× weekly (Mon/Wed/Fri) for testicular maintenance during TRT.

Easy measuring: At 2,500 IU/mL, 1 unit = 25 IU on a U‑100 insulin syringe.

Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 60 days.

Human Chorionic Gonadotropin (HCG) is a glycoprotein hormone that mimics luteinizing hormone (LH) by binding to LH receptors in the gonads[1]. With a 36‑hour half‑life (compared to LH’s 30 minutes), HCG provides sustained stimulation of testosterone production in men and ovulation induction in women[2]. This educational protocol presents a practical three‑times‑weekly subcutaneous approach for maintaining testicular function and fertility.

Research context: For evidence on mechanisms, human and preclinical research, limitations, and safety, read What Is HCG? A Clear, Evidence-Based Guide to hCG (Human Chorionic Gonadotropin).

Standard Protocol (2 mL = 2,500 IU/mL)

Weeks 1–12

500 IU

20 units (0.20 mL)

Frequency: Inject 3 times weekly subcutaneously (e.g., Monday/Wednesday/Friday). This yields a total weekly dose of 1,500 IU. This schedule maintains intratesticular testosterone during exogenous testosterone therapy and supports fertility preservation[3][4].

Reconstitution Steps

Draw 2.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming or vigorous shaking.

Gently swirl or roll until the powder fully dissolves (clear solution).

Label vial with reconstitution date and concentration (2,500 IU/mL); refrigerate immediately at 2–8 °C (35.6–46.4 °F).

Dosing Calculations:

250 IU = 10 units (0.10 mL)

500 IU = 20 units (0.20 mL)

1,000 IU = 40 units (0.40 mL)

High‑Dose Protocol (for Post‑Cycle Recovery or Severe Suppression)

Weeks 1–4

1,500 IU

60 units (0.60 mL)

Weeks 5–8

2,000 IU

80 units (0.80 mL)

Weeks 9–12

1,000 IU

40 units (0.40 mL)

Frequency: Inject 3 times weekly subcutaneously. High‑dose protocols (1,500–2,500 IU per injection) are used to reactivate testosterone production after prolonged anabolic steroid use or severe hypogonadotropic hypogonadism[5][6]. After initial recovery, doses are typically reduced to maintenance levels (500–1,000 IU 3×/week).

Supplies Needed

Plan based on an 8–16 week protocol using standard 500 IU × 3 times weekly dosing.

Peptide Vials (HCG, 5000 IU each):

8 weeks (1,500 IU/week × 8 = 12,000 IU total): 3 vials

12 weeks (1,500 IU/week × 12 = 18,000 IU total): 4 vials

16 weeks (1,500 IU/week × 16 = 24,000 IU total): 5 vials

Insulin Syringes (U‑100):

Per week: 3 syringes (Mon/Wed/Fri)

8 weeks: 24 syringes

12 weeks: 36 syringes

16 weeks: 48 syringes

Bacteriostatic Water (10 mL bottles): Use 2.0 mL per vial for reconstitution.

8 weeks (3 vials): 6 mL → 1 × 10 mL bottle

12 weeks (4 vials): 8 mL → 1 × 10 mL bottle

16 weeks (5 vials): 10 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each injection day.

Per week: 6 swabs (2 per injection × 3 injections)

8 weeks: 48 swabs → recommend 1 × 100‑count box

12 weeks: 72 swabs → recommend 1 × 100‑count box

16 weeks: 96 swabs → recommend 1 × 100‑count box

Connected reading

Helpful context for this guide

Source-derived material selected through this article’s indexed topics.

Related questions

01Humanin — frequently asked questions

Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units. There is no single correct amount — more water simply spreads the same 5 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units. On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand. Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product. Divide the vial strength of 5 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose. No. Humanin is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.

Source: dosagepeptide.com ↗
02GHK (Copper-Free) — frequently asked questions

Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units. There is no single correct amount — more water simply spreads the same 50 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units. On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand. Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product. Divide the vial strength of 50 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose. No. GHK (Copper-Free) is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.

Source: dosagepeptide.com ↗
03Pemvidutide — frequently asked questions

Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units. There is no single correct amount — more water simply spreads the same 5 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units. On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand. Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product. Divide the vial strength of 5 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose. No. Pemvidutide is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.

Source: dosagepeptide.com ↗
04VK2735 — frequently asked questions

Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units. There is no single correct amount — more water simply spreads the same 10 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units. On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand. Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product. Divide the vial strength of 10 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose. No. VK2735 is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.

Source: dosagepeptide.com ↗
05IGF-1 DES — frequently asked questions

Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units. There is no single correct amount — more water simply spreads the same 1 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units. On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand. Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product. Divide the vial strength of 1 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose. No. IGF-1 DES is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.

Source: dosagepeptide.com ↗
Research context

Read sources and limitations before applying a claim.

Additional Research and Background

These sources provide related context and are not presented as support for a specific statement above. Journal of Medicinal Chemistry (ACS) — Bisubstrate inhibitors of NNMT with enhanced activity View Source Related research, protocols, and guides Explore the available research context, protocol variants or comparisons, and practical guides. When vial-size variants exist, they remain separate because vial strength, concentration, and syringe-unit calculations can differ. Related compounds and blends are comparisons only, not interchangeable. Research overview 5-Amino-1MQ Peptide: Benefits, Uses, Side Effects, Dosage, and Research Other vial-size protocols 5-Amino-1MQ (50 mg Vial) Dosage Protocol Practical guides Single Peptide Dosages Peptide Dosage & Reconstitution Calculator How to Reconstitute Peptides Syringe & Measurement Guide Peptide Storage Guide Peptide Dosage Chart

Source: peptidedosages.com ↗

Handling in a Research Context

Because Sermorelin is most often encountered as a lyophilized (freeze-dried) powder in a sealed vial, a brief, strictly educational note on laboratory handling is warranted — with the emphasis that this describes standard research-peptide practice, not a usage recommendation, and that Sermorelin is not an approved therapeutic for aging, body composition, or cognition. Lyophilized peptides are generally reconstituted with sterile or bacteriostatic water for laboratory purposes. The diluent is directed slowly against the inside wall of the vial rather than sprayed onto the powder, and the vial is gently swirled rather than shaken, because vigorous agitation can shear peptide bonds and denature the material. Sermorelin is a relatively delicate molecule — its DPP-4-vulnerable N-terminus that makes it short-lived in blood also makes the reconstituted peptide sensitive to degradation, so cold storage and minimal freeze-thaw cycling matter more than for hardier peptides.3 The volume of diluent chosen simply sets the concentration: a fixed mass dissolved in a larger volume yields a lower concentration per unit volume, the arithmetic behind any reconstitution chart. Lyophilized storage Cold and dark; long-term stability favored by freezing After reconstitution Refrigerated; used within a limited window given peptide fragility Light and heat Minimize exposure; both accelerate peptide breakdown Agitation Swirl gently; avoid shaking or foaming Freeze-thaw Repeated cycles degrade peptides; avoid Sterility Aseptic technique; bacteriostatic water for multi-use practice Meticulous handling changes nothing about the evidence questions raised above. A perfectly reconstituted, high-purity vial of Sermorelin still faces every open question about long-term benefit and safety in non-deficient adults. Good technique preserves whatever biological activity the molecule has; it does not create efficacy where none has been demonstrated.

Source: dosagepeptide.com ↗
Practical and safety references

These excerpts are educational, not personalised medical instructions.

How-to reference

How to convert mcg to mg (and back)

Because the factor is exactly 1000, every conversion is a decimal-point move of three places — no calculator strictly required once you see the pattern: mcg → mg: divide by 1000, i.e. move the decimal point three places to the left. 500 mcg → 0.5 mg; 100 mcg → 0.1 mg; 1500 mcg → 1.5 mg. mg → mcg: multiply by 1000, i.e. move the decimal point three places to the right. 0.5 mg → 500 mcg; 2 mg → 2000 mcg; 1.25 mg → 1250 mcg. The tool above does the same move for you and trims trailing zeros, so you can paste in any value — whole or fractional — and read the exact counterpart.

Source: dosagepeptide.com ↗
Dosage reference

Dosing & Reconstitution Guide

A single practical dilution with accurate once-daily dosing, step by step Frequency: one subcutaneous injection each day. The 4 mg/day dose and the 1–4–8 mg dose-ranging arms are real Phase 2 parameters[3][4], but Phase 2 results are early-stage and proven efficacy should not be assumed.

Source: dosagepeptide.com ↗
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Peptide Therapy Guide Editorial Team

Editorial team for Peptide Therapy Guide.

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