Educational guide
HCG Dosage Protocol | PeptideDosages.com
HCG (5000 IU Vial) Dosage Protocol HCG Dosage Chart HCG is dosed at 500 IU weekly via subcutaneous injection in educational protocols. Reconstitute the 5000 IU vial with bacteriostatic water before use. This information is for research and educational use only
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HCG (5000 IU Vial) Dosage Protocol
HCG Dosage Chart
HCG is dosed at 500 IU weekly via subcutaneous injection in educational protocols. Reconstitute the 5000 IU vial with bacteriostatic water before use. This information is for research and educational use only.
Reconstitute: Add 2.0 mL bacteriostatic water → 2,500 IU/mL concentration.
Typical dosing: 500 IU subcutaneous, 3× weekly (Mon/Wed/Fri) for testicular maintenance during TRT.
Easy measuring: At 2,500 IU/mL, 1 unit = 25 IU on a U‑100 insulin syringe.
Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 60 days.
Human Chorionic Gonadotropin (HCG) is a glycoprotein hormone that mimics luteinizing hormone (LH) by binding to LH receptors in the gonads[1]. With a 36‑hour half‑life (compared to LH’s 30 minutes), HCG provides sustained stimulation of testosterone production in men and ovulation induction in women[2]. This educational protocol presents a practical three‑times‑weekly subcutaneous approach for maintaining testicular function and fertility.
Research context: For evidence on mechanisms, human and preclinical research, limitations, and safety, read What Is HCG? A Clear, Evidence-Based Guide to hCG (Human Chorionic Gonadotropin).
Standard Protocol (2 mL = 2,500 IU/mL)
Weeks 1–12
500 IU
20 units (0.20 mL)
Frequency: Inject 3 times weekly subcutaneously (e.g., Monday/Wednesday/Friday). This yields a total weekly dose of 1,500 IU. This schedule maintains intratesticular testosterone during exogenous testosterone therapy and supports fertility preservation[3][4].
Reconstitution Steps
Draw 2.0 mL bacteriostatic water with a sterile syringe.
Inject slowly down the vial wall; avoid foaming or vigorous shaking.
Gently swirl or roll until the powder fully dissolves (clear solution).
Label vial with reconstitution date and concentration (2,500 IU/mL); refrigerate immediately at 2–8 °C (35.6–46.4 °F).
Dosing Calculations:
250 IU = 10 units (0.10 mL)
500 IU = 20 units (0.20 mL)
1,000 IU = 40 units (0.40 mL)
High‑Dose Protocol (for Post‑Cycle Recovery or Severe Suppression)
Weeks 1–4
1,500 IU
60 units (0.60 mL)
Weeks 5–8
2,000 IU
80 units (0.80 mL)
Weeks 9–12
1,000 IU
40 units (0.40 mL)
Frequency: Inject 3 times weekly subcutaneously. High‑dose protocols (1,500–2,500 IU per injection) are used to reactivate testosterone production after prolonged anabolic steroid use or severe hypogonadotropic hypogonadism[5][6]. After initial recovery, doses are typically reduced to maintenance levels (500–1,000 IU 3×/week).
Supplies Needed
Plan based on an 8–16 week protocol using standard 500 IU × 3 times weekly dosing.
Peptide Vials (HCG, 5000 IU each):
8 weeks (1,500 IU/week × 8 = 12,000 IU total): 3 vials
12 weeks (1,500 IU/week × 12 = 18,000 IU total): 4 vials
16 weeks (1,500 IU/week × 16 = 24,000 IU total): 5 vials
Insulin Syringes (U‑100):
Per week: 3 syringes (Mon/Wed/Fri)
8 weeks: 24 syringes
12 weeks: 36 syringes
16 weeks: 48 syringes
Bacteriostatic Water (10 mL bottles): Use 2.0 mL per vial for reconstitution.
8 weeks (3 vials): 6 mL → 1 × 10 mL bottle
12 weeks (4 vials): 8 mL → 1 × 10 mL bottle
16 weeks (5 vials): 10 mL → 1 × 10 mL bottle
Alcohol Swabs: One for the vial stopper + one for the injection site each injection day.
Per week: 6 swabs (2 per injection × 3 injections)
8 weeks: 48 swabs → recommend 1 × 100‑count box
12 weeks: 72 swabs → recommend 1 × 100‑count box
16 weeks: 96 swabs → recommend 1 × 100‑count box