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GenCRISPR gRNA & HDR Template Design Tools
GenCRISPR gRNA & HDR Template Design Tools Accelerate Precision Gene Editing Resources » Bioinformatics Tools » GenCRISPR gRNA Design Tool The GenCRISPR gRNA Design Tools makes guide RNA and HDR template design easy – Simply select your experiment type to get
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GenCRISPR gRNA & HDR Template Design Tools
Accelerate Precision Gene Editing
Resources » Bioinformatics Tools » GenCRISPR gRNA Design Tool
The GenCRISPR gRNA Design Tools makes guide RNA and HDR template design easy – Simply select your experiment type to get started.
Note: Selecting HDR knock-in will enable HDR template design options
Why choose our CRISPR design tools?
sgRNA design tool
Comprehensive species options, updated on-target and off-target scores
Designs target early exons to avoid truncated functional proteins
Higher transcript coverage
Ideal GC% for sgRNA
Validated KO% up to 97%, more detail>>
Design Tool User Guide
HDR knock-in template design tool
Supports 1-1500bp deletions, 1bp-20kb insertions
Linear ssDNA / linear closed-end dsDNA / circular dsDNA formats available
CTS option and updated on-target and off-target scores increase editing efficiency
Multiple sequence check function helps avoid mistakes
Base Editing Design Tool
Supports ABE and CBE editing systems
Visualizes sequence and amino acid alignment before and after editing
Provides precise gRNA design with On-Target/Off-Target scoring and validation primer design
pegRNA design tool
Comprehensive Functions: Simply enter your desired gene symbol or sequence for guided support from design to ordering.
More Editing Approaches: Supports knock-ins, knockouts, and sequence replacement, with knock-ins up to 100 nt.
Enhances Editing Efficiency: Includes nicking sgRNA, 3' extension, and silent mutations.
Easy to Use: Displays base and amino acid changes, with multiple indices for easy selection.
How do GenScript’s CRISPR design tools compare to other available design tools?
GenScript CRISPR Design Tools
Synthego CRISPR Design Tool
IDT Alt-R™ CRISPR Design Tools
Editing Systems Supported
Cas9, Cas12a, prime editing
Cas9 only
Multiple mammalian and bacterial species supported
Yes
On-/Off-target Scoring
Advanced
Basic
Edit Types Supported
Knockout, knock-in, sequence replacement
Knockout only
Knockout, knock-in
HDR Template Design Options
Three modalities
None
Two modalities
Plasmid Design Options
No
Designs for Optimized GC Content
Targets Early Exons
Optimized Transcript Coverage
Purification Options
Desalt and HPLC
Desalt only
Easily design compatible HDR templates & sgRNAs
Automatically optimize homology arm design
Select from ssDNA or dsDNA formats
Create an SNP or insert any sequence up to 20 kb
Streamline your HDR template & sgRNA orders
sgRNA/template pairs are ranked by algorithm scores
Interactive Design Map
Paired sgRNA + HDR Templates
How to Design CRISPR Knock-in sgRNA + HDR Templates
Other CRISPR gRNA Design Tool And Database
Broad gRNA Design Tool for Any Sequence
sgRNA designed for any DNA sequence you input
Algorithm developed and validated by Feng Zhang lab, Broad Institute
Broad sgRNA Database
Genome-wide gRNA databases for CRISPR editing and transcription activation
Pre-validated gRNA sequences design by the Broad Institute Ready for delivery; Full-Genome Coverage
GenCRISPR Services
CRISPR/Cas9 sgRNA Synthesis
Homology-Directed Repair (HDR) Knock-in Templates
Accelerate Precision Gene Editing
GenCRISPR™ gRNA/Cas9 Plasmids
Licensed from the Feng Zhang Laboratory at the Broad Institute Reduce Off-Target Effects with Enhanced SpCas9
CRISPR Cell Line Development
Make Genome Editing Easy
CRISPR gRNA Libraries
Genome-wide gRNA libraries for efficient screeningNow in stock and available from just $999 for 25 µg
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