Educational guide
FITC Peptide Labeling
FITC Peptide Labeling FITC is one of the most widely used fluorescent labels for peptides. It is commonly used in fluorescence microscopy, flow cytometry, uptake studies, and general biomolecular tracking applications. Key Properties Excitation / Emission 495
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FITC Peptide Labeling
FITC is one of the most widely used fluorescent labels for peptides. It is commonly used in fluorescence microscopy, flow cytometry, uptake studies, and general biomolecular tracking applications.
Key Properties
Excitation / Emission
495 / 519 nm
Color
Green
Laser Compatibility
488 nm
Typical Use
Microscopy, flow cytometry, uptake studies
Notes
Commonly used with Ahx spacer for peptide labeling
FITC Spectrum
Why Use FITC?
Widely recognized green fluorescent label
Compatible with common 488 nm excitation sources
Useful for peptide tracking and cellular assays
Well established in many antibody and peptide workflows
Peptide Labeling Options
N-terminal FITC labeling
Lysine side-chain labeling
Cysteine-directed conjugation in selected designs
Optional Ahx or β-Ala spacer to reduce steric interference
Related Dyes
FAM
ATTO 488
Alexa Fluor 647
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