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DNA Transformation Protocol
DNA Transformation Protocol Resources » Technical Resource Centers » Gene Technical Resources » Molecular Cloning Center » DNA Transformation Protocol Molecular Cloning Strategies What is PCR Restriction Enzymes Digestion DNA Ligation Plasmid Transformation DN
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DNA Transformation Protocol
Resources » Technical Resource Centers » Gene Technical Resources » Molecular Cloning Center » DNA Transformation Protocol
Molecular Cloning Strategies
What is PCR
Restriction Enzymes Digestion
DNA Ligation
Plasmid Transformation
DNA Transfection Guide
Transfection Protocol
DNA Transfection Troubleshooting
Ligation Protocol
DNA Ligation Troubleshooting Guide
PCR Protocol, PCR Steps
PCR Troubleshooting Guide
Restriction Digest Protocol
Restriction Digestion Troubleshooting Guide
DNA Ligation Troubleshooting
What is DNA Transformation|Biology
DNA Transformation Protocol
Transformation Troubleshooting Guide
Molecular cloning strategies
PCR
Restriction digestion
Ligation
Transformation
Transfection
Molecular cloning handbook
Bioinformatics tools
Thaw all reagents completely on ice.
Add 1 µL of ligation reaction to thawed competent cells.
Gently mix by tapping tube of competent cells.
Incubate reaction on ice for 30 minutes.
Heat shock the competent cell mixture by incubation for 30 to 60 seconds in a 42°C water bath.
Incubate tubes on ice for 2 minutes.
Add 250 to 500 µL of SOC or LB media.
Incubate at 37°C and shake at 250 rpm.
Warm selection plates to 37°C.
Spread 10, 50, and 100 µL of transformed cells on selection plates.
Incubate plates at 30°C overnight.
Competent cells
to 50 µL
Ligation reaction
1-5 µL
SOC or LB media
950 ml
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