Cyclic Peptides Synthesis

Cyclic Peptides Synthesis

Targeted Drug Delivery Peptide Therapeutics Development Cell-penetrating Peptide Conjugation Advanced Cyclization Techniques

Creative Peptides provides a wide range of cyclic peptide synthesis, which play a key role in different processes and have good therapeutic potential because they have several favorable properties, such as displaying a large surface area, which leads to high affinity and selectivity for protein targets, as well as their limited conformational flexibility, reducing entropy penalties during binding, thus improving their binding performance.

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Cyclic Peptides Synthesis Methods

Depending on the cyclization position, there are several methods to synthesize cyclic peptides: head-to-tail, side-chain-to-side-chain, head-to-side-chain, and side-chain-to-tail, head-to-backbone, backbone-to-tail, side-chain-to-backbone, backbone-to-side-chain. (see figure below). While head-to-tail cycles are usually formed by amide bond formation, side-chain-to-side-chain cycles are most often synthesized via Cys-Cys or amide bond formation.

Backbone Cyclization

Backbone cyclic peptides are easily assembled via the formation of an amide bond between the peptides own N-terminus amine and C-terminus carboxylic acid. Our commonly used methods are chemical synthesis and biosynthesis. Creative Peptides perform the reaction with very high yields, and routinely provide over 98% purified cycled peptides.

Side-chain Cyclization

Side chain-to-side chain cyclization is frequently employed to stabilize and select specific conformations and to reduce susceptibility toward proteolytic degradation. Creative Peptides have the capable to selectively protect, and then deprotect peptide sidechains to allow for the formation of lactam bridges between Lysine or Diaminopropanoic acid, and Glutamic and Aspartic Acids. Creative Peptides can even perform multiple different sites for bridge formation.

Disulfide-rich Peptides

Disulfide bonds are widely present in hormones, enzymes, and immunoglobulins, and are thought to play an important role in reconstructing the conformation of biologically active peptides. To further enhance biological activity, people often introduce disulfide bonds to modify bioactive peptides. Creative Peptides usually add two cysteine residues to the peptide sequence, and the dilute solution of the peptide undergoes mild oxidation to form the desired intramolecular disulfide bond between the two cysteine residues. Ugi reaction also can be provided.

Hydrocarbon-Stapled Peptide

Disulfide bonds can be easily reduced to their acyclic mercaptan form in the intracellular environment. This challenge can be overcome by hydrocarbon-bound peptide synthesis, which can form a stable α-helix structure. It can mimic the typical molecular structure at the protein-protein interaction interface. When locked in this stable configuration, the constrained peptides can penetrate the cell and act on the protein targets in the cell.

Cyclic Peptides Synthesis Services

Custom Cyclic Peptide Synthesis

We offer custom cyclic peptide synthesis for a variety of applications, from basic research to complex therapeutic peptides. Our team ensures high purity, efficiency, and flexibility in the synthesis process, enabling the production of peptides that meet your precise specifications.

Full-Length Cyclic Peptides

Small and Large Peptides

Tailored Modifications and Conjugations

High-Throughput Cyclic Peptide Synthesis

Accelerate your research with our high-throughput cyclic peptide synthesis services. We employ automated systems to produce large quantities of peptides quickly and efficiently, making it ideal for screening campaigns and large-scale projects.

Fast Turnaround Times

Automation for Scalability

ARobust Quality Control

Peptide Cyclization Techniques

Our peptide cyclization techniques include a range of methods for efficient ring formation, ensuring that the cyclic peptides are stable and functional. We use both chemical and enzymatic cyclization strategies to produce peptides with diverse ring structures.

Head-to-Tail Cyclization

Disulfide Bond Formation

Stapled Peptides

Peptide Modification and Labeling

Enhance your cyclic peptides with various modifications such as fluorescent labeling, biotinylation, and PEGylation for improved bioavailability, stability, and detection. Our modification services are ideal for peptide-based assays and diagnostic applications

Fluorescent Tags

Biotinylation

PEGylation for Enhanced Solubility

Peptide Library Construction

We provide cyclic peptide library construction services to help you explore diverse peptide sequences. These libraries are invaluable for drug discovery, biomarker identification, and functional screening.

Diverse Sequence Variations

High-Density Libraries

Screening for Target Interaction

Peptide Purification & Characterization

We use advanced techniques to purify and characterize your cyclic peptides to ensure they meet the highest standards. Our services include HPLC purification, mass spectrometry, and NMR analysis to confirm peptide identity, purity, and structure.

HPLC Purification

Mass Spectrometry

NMR Structural Analysis

Peptide Therapeutics Development

Explore the therapeutic potential of cyclic peptides with our specialized services for drug development. From initial synthesis to preclinical studies, we assist in developing peptide-based therapeutics for cancer, autoimmune diseases, neurodegenerative diseases, and more.

Targeted Drug Delivery

Anticancer Peptides

Antimicrobial Cyclic Peptides

Contract Research and Collaboration

Partner with us for contract research and collaborative projects. Whether you're a biotech company, pharmaceutical firm, or academic institution, we offer comprehensive support for your cyclic peptide research needs.

Flexible Collaboration Models

Confidentiality Assured

Custom Research Solutions

TAT Peptide Synthesis

We specialize in TAT peptide synthesis, offering high-quality peptides for drug delivery, gene therapy, and targeted cellular interactions. Our expertise ensures efficient and reliable synthesis with optimal purity and bioactivity, ideal for your research and therapeutic applications.

DOTAGA Chelating Peptide Synthesis

Our DOTAGA chelating peptide synthesis service provides custom peptides with DOTAGA (1,4,7,10-Tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid) for use in radiolabeling and molecular imaging. With precise synthesis techniques, we ensure high-quality peptides for applications in diagnostic imaging and targeted therapies.

Synthesis of Head-to-Tail Cyclic Peptides

We offer head-to-tail cyclic peptide synthesis, a method that forms stable cyclic peptides through reliable chemical or enzymatic cyclization techniques. Our service ensures robust peptide structures with enhanced stability, ideal for drug discovery and biomolecular research.

Bicyclic Peptides Synthesis

Our bicyclic peptides synthesis service delivers peptides with two interconnected rings, enhancing stability and bioactivity. By utilizing advanced cyclization techniques, we provide highly potent peptides suitable for therapeutic, diagnostic, and bioengineering applications.

Key Technologies and Platforms for Cyclic Peptides Synthesis

Solid-Phase Peptide Synthesis (SPPS)

We use solid-phase peptide synthesis (SPPS), a widely recognized technique that allows for the efficient assembly of cyclic peptides, ensuring high purity and high yields throughout the synthesis process.

High-Performance Liquid Chromatography (HPLC)

To ensure the highest peptide purity, we employ HPLC for precise peptide purification, removing any impurities and ensuring that the final product meets your exact specifications.

Mass Spectrometry (MS)

We utilize mass spectrometry (MS) to accurately confirm the molecular weight and structure of the synthesized cyclic peptides, providing essential data for quality control and verification.

Nuclear Magnetic Resonance (NMR) Spectroscopy

Our NMR spectroscopy platform allows us to verify the structural integrity of cyclic peptides, ensuring that each molecule maintains its intended conformation for optimal performance in your applications.

Automated Peptide Synthesizers

For high-throughput peptide synthesis, we rely on automated peptide synthesizers, which streamline the production of large quantities of peptides with consistent quality and minimal turnaround time.

Peptide Modification Platforms

Our peptide modification platforms enable the efficient addition of functional groups, such as fluorescent labels, biotinylation, and PEGylation, enhancing the peptide's bioavailability, stability, and targeting ability.

Why Choose Us for Cyclic Peptide Synthesis?

Experienced Peptide Synthesis Experts

Our team of specialists brings years of expertise in cyclic peptide synthesis, ensuring high-quality results.

State-of-the-art Facilities and Equipment

We utilize the latest technology and equipment to deliver precise and reliable peptide synthesis.

Competitive Pricing

We offer cost-effective solutions without compromising on quality, making our services accessible for all project sizes.

Exceptional Quality Control and Testing

Every peptide we synthesize undergoes rigorous testing to guarantee purity and functionality.

Global Client Base with Proven Results

We have successfully delivered custom cyclic peptides to clients worldwide, ensuring high customer satisfaction and trust.

Scalability

From small-scale to high-throughput peptide synthesis, we provide flexible solutions for any project size.

Cyclic Peptides Synthesis Workflow

1

Initial Consultation

We begin with a detailed consultation to understand your specific needs, including peptide sequence, modifications, and application requirements.

2

Custom Peptide Design

Our experts work with you to design a custom cyclic peptide that fits your research goals, ensuring the peptide sequence and cyclization method are optimized for stability and bioactivity.

3

Peptide Synthesis

Using solid-phase peptide synthesis (SPPS), we efficiently assemble your cyclic peptide, ensuring high purity and yield throughout the process.

4

Peptide Purification & QC

We employ HPLC purification and mass spectrometry (MS) to thoroughly purify and confirm the identity and structure of your cyclic peptide, ensuring it meets the highest quality standards.

5

Peptide Modification (if needed)

If necessary, we add functional groups such as fluorescent labeling, biotinylation, or PEGylation to enhance the peptide's functionality for applications like drug delivery or diagnostics.

6

Delivery & Support

Once your peptide is synthesized, purified, and modified, we provide fast delivery and ongoing support, ensuring it meets your research or development timelines.

Application Fields of Cyclic Peptides

Drug Discovery and Development

Cyclic peptides have emerged as valuable tools in drug discovery, offering unique properties such as enhanced stability and target specificity. Our cyclic peptide synthesis services help researchers create potent peptide-based drugs, enabling better targeting of diseases like cancer, infections, and autoimmune disorders. By utilizing advanced cyclization techniques, we can develop peptides with improved pharmacokinetics and therapeutic efficacy, which are crucial for the creation of next-generation therapeutics.

Cancer Therapy

Cyclic peptides play a pivotal role in cancer therapy by targeting specific tumor markers and cancer cells with high precision. Our synthesis services focus on creating cancer-targeting cyclic peptides that are able to bind to tumor receptors with high affinity. These peptides are being increasingly used in the development of anticancer drugs and immunotherapies, improving the specificity and reducing side effects compared to conventional therapies.

Diagnostic Imaging

In the field of molecular imaging, cyclic peptides are often used as targeting agents for diagnostic applications, such as PET (Positron Emission Tomography) and MRI. Our DOTAGA chelating peptide synthesis service ensures the synthesis of peptides that can be radiolabeled, allowing for precise imaging of tissues and organs. These peptides enable researchers and clinicians to monitor disease progression, identify abnormalities, and improve the accuracy of early diagnoses.

Vaccine Development

Cyclic peptides have shown great promise in vaccine development, particularly for peptide-based vaccines targeting specific pathogens or diseases. By synthesizing immunogenic cyclic peptides, we help researchers create vaccines with high stability and immunogenicity. These vaccines are not only more stable at room temperature but also capable of triggering stronger and longer-lasting immune responses.

Drug Delivery Systems

One of the significant advantages of cyclic peptides is their ability to enhance drug delivery systems. Due to their stability and ability to penetrate cell membranes, cyclic peptides are increasingly used as carriers for delivering drugs, proteins, and genes directly to target cells. Our synthesis services can provide TAT peptides and other delivery peptides for targeted therapy, improving the efficiency and reducing side effects of treatments.

Antimicrobial Peptides

Cyclic peptides are also being explored as alternatives to traditional antibiotics in the fight against drug-resistant bacteria and infections. Our antimicrobial cyclic peptide synthesis services focus on creating peptides with strong antimicrobial properties that are effective against a broad range of pathogens. These peptides can be used in the development of antibiotic therapies that overcome the growing threat of antimicrobial resistance.

Biomolecular Research

Cyclic peptides are widely used in biomolecular research to study protein-protein interactions, enzyme activity, and cellular signaling pathways. Our cyclic peptide synthesis services offer high-quality peptides that can be used as tools for research on gene expression, protein binding, and signal transduction, enabling scientists to explore complex biological systems in greater detail.

Other Services You May Need

Peptide Modifications & Labels

Creative Peptides also has extensive experience in the design and production of modified peptides. We provide a variety of peptide modifications, labeling, and bioconjugation with other biomolecules, including

Peptide N-Terminal Modification

Peptide C-Terminal Modification

Peptide PEGylation

Biotinylated Peptides

Fluorescence and Dye Labeling Peptides

Post-translational Modification

Custom Labeling

Peptide Tags

Unusual and Non-natural Amino Acids Modification

Peptide Analysis Services

In addition, we also provide a variety of optional peptide analysis, including

Surface Plasmon Resonance Imaging (SPRi)Service

Epitope Mapping Services

Peptide Characterization

Peptide Library and Array

Biological Testing

Chemical & Physical Analyses

Peptide Conjugations

Creative Peptides provides one-stop peptide solutions, from peptide synthesis, modification, labeling and conjugation to a wide range of biopolymers and surface attachments. Our peptides conjugation services include, but are not limited to

Gold/Silver Nanoparticles Conjugation Service

Peptides-Carraier Protein Conjugation

Peptide-oligonucleotide Conjugation

Peptide-enzyme Conjugation

Peptides-Metal Chelates Conjugation

NHS-Activated Peptide Conjugation Magnetic Beads

Peptides-Imaging Agents Conjugation

Peptides-Fatty Acids Conjugation

Peptide-small-molecule Conjugation

Peptide-Antibody Conjugation

Peptide-siRNA Conjugation

Peptide-DNA Conjugation

Peptide-RNA Conjugation

Explore Our Product Range

CAT#

Product Name

M.W

Molecular Formula

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10-101-103

Vancomycin

1449.25

C66H75Cl2N9O24

Inquiry

10-101-104

Teicoplanin

1879.66

C88H95Cl2N9O33

10-101-169

Pasireotide

1047.2

C58H66N10O9

10-101-186

Romidepsin

540.69584

C24H36N4O6S2

10-101-325

Semaglutide

4113.57

C187H291N45O59

10-101-62

Ziconotide

2639.2

C102H172N36O32S7

10-101-78

Dalbavancin

1816.69

C88H100Cl2N10O28

AF083

Polymyxin B

MFP-041

Rezafungin

1226.4

C63H85N8O17

R04030

Cyclo(-Arg-Gly-Asp-D-Phe-Val)

574.6

C26H38N8O7

R1574

Octreotide

1019.2

C49H66N10O10S2

R1812

Lanreotide

1096.3

C54H69N11O10S2

R1824

Cyclo(RGDyK)

847.7

C31H43F6N9O12

R2018

Capreomycin

R2029

Enviomycin

685.69

C26H43N11O11

R2052

Zilucoplan

3562

C172H278N24O55

R2238

Telavancin

1755.6

C80H106Cl2N11O27P

R2239

Oritavancin

1793.1

C86H97Cl3N10O26

R2240

Bacitracin

1422.69

C66H103N17O16S

R2241

Viomycin

C25H43N13O10

R2242

Colistin

1169.5

C53H100N16O13 (for E1)

R2243

Micafungin

1270.27

C56H71N9O23S

R2244

Anidulafungin

1140.24

C58H73N7O17

R2245

Fostamatinib

580.46

C23H26FN6O9P

R2246

Paritaprevir

765.88

C40H43N7O7S

R2247

Grazoprevir

766.9

C38H50N6O9S

Z10-101-154

Daptomycin

1620.69

C72H101N17O26

Z10-101-157

Caspofungin

1093.31

C52H88N10O15

Boost Your Research with These Insights

FAQs

Cyclic peptide synthesis is the process of creating peptides that form a ring structure, which enhances their stability, bioactivity, and specificity. This technique is widely used for applications in drug discovery, cancer therapy, diagnostic imaging, and more.

Cyclic peptides offer enhanced stability compared to linear peptides, making them more effective for therapeutic use. Their unique structure also allows for higher affinity binding to target molecules, which is especially beneficial in drug discovery, cancer therapy, and diagnostic imaging.

Yes, we offer fully customizable cyclic peptide synthesis. Whether you need peptides for drug development, targeted drug delivery, or biomolecular research, we tailor the synthesis process to meet your exact needs, including modifications like fluorescent labeling, biotinylation, and PEGylation.

We employ rigorous quality control measures, including HPLC purification, mass spectrometry, and NMR analysis to verify the identity, purity, and structure of each peptide. This ensures that our cyclic peptides meet the highest standards for scientific and therapeutic use.

Absolutely! We provide high-throughput cyclic peptide synthesis services to accommodate large-scale production needs, ideal for screening campaigns, peptide libraries, and extensive research studies. Our automated systems ensure consistent quality and rapid delivery for large quantities.