Custom Bridged Nucleic Acid Synthesis

Custom Bridged Nucleic Acid Synthesis

As a pioneer in oligonucleotide synthesis, Creative Peptides has been working on developing new oligonucleotide-based technologies that can produce compounds with excellent binding affinity and chemical/biological stability. Creative Peptides has advanced equipment, advanced technology and experienced staff to help you synthesize bridged nucleic acid (BNA) oligodeoxynucleotides of interest to ensure your satisfaction in a timely and professional manner.

What is Bridged Nucleic Acids (BNA)?

Bridged nucleic acids (BNA) are modified RNA nucleotides, sometimes referred to as restricted or inaccessible RNA molecules. BNA monomers can contain five-membered, six-membered or even seven-membered bridging structures with "fixed" C3'-endo sugar puckering. The chemical structure of the BNA monomer contains a bridge at the 2',4'-position of ribose to provide the 2',4'-BNA monomer synthesized by the Takeshi Imanishi team. The properties of the bridge can vary according to different types of monomers. BNA monomers can be easily integrated into natural oligonucleotides by standard phosphite amide chemistry, and BNA/DNA and BNA/RNA heterozygous oligonucleotides can be flexibly designed to meet the requirements of high and sequence-specific hybridization with natural nucleic acids and strong nuclease resistance.

So far, the third generation BNA has been developed. 4'-BNA-NC contains a 6-membered ring with a NO bond between the 2'-OH and the 4'-C part of the ribose. It has excellent binding affinity to ssRNA, better binding affinity to dsDNA and excellent enzyme stability to nuclease. As a result, BNA has now become an excellent tool for developing high-value testing systems and therapeutic products. In addition, newer BNA compounds will be introduced in the near future, which brings great hope for the research and application fields based on oligonucleotides.

Advantage of Bridged Nucleic Acid

BNA has higher binding affinity to their complementary chains and excellent ability to distinguish single mismatch.

Stronger and more sequence-selective triplex formation characteristics and higher nuclease resistance.

Compared with traditional DNA or RNA oligonucleotides, it has better water solubility.

Compared with peptide nucleic acid (PNA), BNA allows better base pair stacking and high stability.

Compared with PNA and LNA, BNA improves hybridization selectivity and specificity.

BNA is an ideal choice for the detection of short RNA and DNA targets.

BNA can increase thermal stability of duplexes and triplexes.

Flexible probe designs regardless of GC content.

BNA has superior antisense inhibition and potency.

Application of Bridged Nucleic Acid

Chromosomal FISH

SNP detection/allele specific PCR

Antisense research

RNAi research

Comparative genome hybridization

In situ hybridization (FISH probe)

RNA function inhibition research (ASO, inhibitor, antagomir, etc.)

Real-time PCR and/or other specific PCR

Antigene inhibition

In vivo, in vitro delivery

Biosensor

Other nucleotide based applications

Our Services

RNase-Free HPLC purification.

Customized third-generation BNA.

Different scales for all customized BNA.

One-stop customized BNA synthesis services from primer design and synthesis to final result delivery.

Different labeling, such as fluorescent dyes, biotin, amino-linkers, etc.

Different modifications for all customized BNA, such as phosphorylation, thiolation, modified bases, linkages, etc.

FAQs

Bridged Nucleic Acid (BNA) is a modified RNA nucleotide with a unique bridge at the 2',4'-position of ribose. This modification enhances the stability, binding affinity, and nuclease resistance, making it ideal for high-specificity hybridization and the development of advanced nucleic acid-based applications.

BNA offers superior binding affinity, enhanced mismatch discrimination, and better nuclease resistance compared to traditional DNA or RNA. It also has stronger triplex formation capabilities and improved thermal stability for duplexes and triplexes.

Unlike PNA and LNA, BNA enhances base pair stacking, ensuring higher hybridization specificity and selectivity. It offers better water solubility and stability, making it a more effective choice for sensitive nucleic acid detection.

BNA is widely used in chromosomal FISH, SNP detection, antisense research, RNAi, in situ hybridization, real-time PCR, and biosensor development, among other nucleic acid-based applications.

BNA improves hybridization selectivity, especially for short RNA and DNA targets, due to its higher thermal stability and sequence-specific binding. This makes it an ideal tool for sensitive detection systems.

We offer custom modifications for BNAs, including phosphorylation, thiolation, fluorescent dye labeling, biotin conjugation, amino-linkers, and more. These modifications enable flexible probe design for a variety of applications.

References

Soler-Bistué, A., Zorreguieta, A., & Tolmasky, M. E. (2019). Bridged nucleic acids reloaded. Molecules, 24(12), 2297.

Kim, S. K., Linse, K. D., Retes, P., Castro, P., & Castro, M. (2015). Bridged nucleic acids (BNAs) as molecular tools. Journal of Biochemistry and Molecular Biology Research, 1(3), 67-71.

Mangla, P., Olety, B., & Sharma, V. K. (2021). Advances in the Synthesis and Antisense Technology Applications of Bridged Nucleic Acid Monomers. Current Organic Chemistry, 25(20), 2475-2498.