CRISPR/Cas Proteins

CRISPR/Cas Proteins

Catalog and custom nucleases for effective gene editing across a variety of systems

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Overview

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) has transformed biological research and therapeutic development. Different Cas proteins offer unique editing capabilities and targeting requirements, directed by the guide RNA to the targeted sequences.

As a leading provider of gene editing technology and partner of the Broad Institute, GenScript offers one-stop solution of Cas protein including a broad portfolio of off-the-shelf products and customized services, with cGMP expertise and global IND track records, to provide researchers an integrated CRISPR workflow solution, accelerate clinical entry and commercialization.

Cas Protein Ready to Use

Spanning across Cas9, Cas12a, MAD7, Cas13a, and next-generation Base Editors and Prime Editors - engineered for high efficiency, low off-target rates, and broad cell type compatibility. Available in both RUO and cGMP formats. Check out >>

Cas Protein Custom Service

Our custom Cas protein service delivers RUO to cGMP nucleases with industry leading speed, scalable manufacturing capacity, comprehensive assay development capability and IND regulatory support to accelerate therapeutic development.

Backed by global compliant QC standards and expert regulatory support, our GMP grade Cas proteins have supported seven IND approvals to date, with FDA DMF activated and ready for IND application.

Need help deciding which Cas enzyme to use?

Target

DNA

Cleavage

Doublestrand break, blunt

Doublestrand break, staggered

Singlestrand break

Single-strand break

Suggested Application

Highly efficient KO/KI

High fidelity, low off-target editing

Multiplex editing, DNA detection

RNA cleavage and detection

Single-base substitutions, short insertion/deletions

Single-base substitutions (A->T, C->G)

Cas9 sgRNA

Prime Editing gRNA

Base Editing gRNA

Cas12a crRNA

cGMP gRNA

GenExact ssDNA

GenWand dsDNA

GenCircle dsDNA

cGMP ssDNA/dsDNA

Non-viral CAR-T Knock-in Optimization Kits

sgRNA Design Tool

HDR Design Tool

pegRNA Design Tool

cGMP Cas Proteins

gRNA List

gRNA Libraries

Cell Engineering

Microbial Editing

Cas9 Nucleases

Catalog Proteins

Off-the-shelf Cas proteins licensed from the Broad Institute.

Next-gen editors

First-in-market Prime and Base editors

High-fidelity

Low off-target eSpCas9 for enhanced specificity

High Purity & Quality

Over 90% purity to ensure reliability and consistency

Regulatory Track Record

7 global IND approvals supported to date DMF on file with FDA

Cas9 Nucleases

SpCas9 (Streptococcus pyogenes Cas9) is a widely used RNA-guided endonuclease in CRISPR Cas9 genome editing, recognizing a 5'-NGG-3' PAM sequence to create blunt-ended doublestrand breaks 3 bp upstream of the PAM. Cas9 nuclease variants including high-fidelity and wild-type versions, are provided for precise, efficient genome editing across a wide range of research and therapeutic applications. Most of the Cas9 protein products are equipped with NLS to facilitate high editing efficiency in eukaryocytes, while the non-NLS Cas9 provides the efficient tool for microbial genome editing. Click on each product link to learn more.

Benefit advanced low-off-target eSpCas9 and no-tag design for high-precise therapeutic developmen

Choose from variant NLS for wild type SpCas9

Streamline fluorescent cell tracking and sorting post transfection with EGFP

Reduce off-target with D10A Nickase and dual gRNA design

High fidelity eSpCas9

Z03622

GenCRISPR™ Ultra eSpCas9-2NLS-Research

$765.00 0.5 mg 1 mg 5 mg

Wild type SpCas9

RC00001

GenCRISPR™ NLS-wtSpCas9, his-tag

$150.00 0.1 mg 1 mg 3 mg

Z03386

GenCrispr Cas9 Nuclease

$35.00 10 μg (0.2mg/ml) 50 μg (5×10μg)(0.2mg/ml)

Z03621

GenCRISPR™ Ultra NLS-Cas9-Research

$695.00 0.5 mg 1 mg 5 mg

Z03702

GenCRISPR™ Cas9 v1.2

$188.00 100 μg 500 μg 1 mg

EGFP SpCas9

Z03393

GenCrispr NLS-Cas9-EGFP Nuclease

$188.00 50 μg (1mg/ml) 100 μg (3mg/ml)

Z03467

$241.00 100 μg(10mg/ml) 500 μg(10mg/ml) 1 mg(10mg/ml)

SpCas9 Nickase

Z03390

GenCrispr NLS-Cas9-D10A Nickase

$39.00 10 μg (1mg/ml) 50 μg (1mg/ml) 100 μg (4mg/ml)

SaCas9

Z03699

GenCRISPR™ SaCas9 2NLS Nuclease

$188.00 0.1 mg 0.5 mg 1 mg

Explore cGMP Cas9 products for seamless scale-up to clinical development

Cas12a Nucleases

Cas12a (formerly Cpf1) is a Class 2 Type V CRISPR-Cas protein used for precise genome editing and nucleic acid detection. Unlike Cas9, Cas12a recognizes T-rich PAM sequences, offering editing in regions beyond Cas9 reach. They also produce staggered cuts and enable higher editing accuracy. Cas12a can process their own guide RNA array and thus is favored for multiplex editing. Cas12a nucleases also exhibit collateral ssDNA cleavage, enabling highly sensitive diagnostic applications on DNA samples. AsCas12a, LbCas12a and ErCas12a (MAD7) are all members of the Cas12a (Cpf1) CRISPR nuclease family with different origins and PAM preference. Click on each product link to learn more.

MAD7

RC00012

GenCRISPR™ Ultra ErCas12a-3NLS, His-tag

$196.00 0.1 mg 0.5 mg 1 mg 5 mg

Z03762

GenCRISPR™ ErCas12a Nuclease

$189.00 100 μg 500 μg 1 mg

AsCas12a

Z03502

GenCRISPR™ Cas12a (Cpf1) Nuclease

$113.00 50 μg 100 μg 500 μg 1 mg

LbCas12a

Z03753

GenCRISPR™ LbCas12a Nuclease

$189.00 100 μg 500 μg 1000 μg

Cas13a Nucleases

Cas13a nucleases are Class 2 Type VI CRISPR-Cas enzyme that acts as an RNA-guided, RNAtargeting ribonuclease. Upon recognizing its target RNA, Cas13a activates a unique "collateral cleavage" mechanism, non-specifically degrading nearby RNA molecules. This collateral activity is harnessed for ultra-sensitive, programmable molecular diagnostics for RNA based diagnostics like pathogen detection. LwaCas13 and LbuCas13 are from different origins, while both exhibit high efficiency and offered at 4mg/ml, and LbuCas13 as the tag-free option. Click on each product link to learn more.

Z03486

GenCRISPR™ Cas13a (C2c2) Nuclease

Z03742

GenCRISPR™ LbuCas13a Nuclease

Prime Editors and Base Editors

Beyond nuclease-based editing, base and prime editors have emerged as nextgeneration genome engineering technologies that enable higher-precision modifications without introducing double-strand breaks (DSBs). By avoiding DSBs, these systems reduce editing-associated errors such as indels and chromosomal rearrangements, resulting in more predictable outcomes.

Base editors typically combine a catalytically impaired Cas protein, such as D10A nickase, with a deaminase to achieve targeted single-base conversions. Adenine base editors (ABEs) mediate A→G substitutions, while cytosine base editors (CBEs) enable C→T conversions. Prime editors pair a Cas nickase with a reverse transcriptase and a prime editing guide RNA (pegRNA) to install a broader range of precise edits, including substitutions, insertions, and deletions, with reduced dependence on donor templates.

Together, these features expand the versatility of genome editing and enable a wider range of therapeutic and research applications.

Base Editors

RC00010

ABE8e

$200.00 30 µg (3mg/ml) 100 μg(10mg/ml) 500 μg(10mg/ml) 5 mg(10mg/ml)

RC00011

CBE4

- 30 μg 100 μg 500 μg 5 mg

Prime Editors

RC00006

PE6b

$200.00 30 µg (3mg/ml) 100 μg(10mg/ml) 500 μg(10mg/ml) 5 mg (10mg/ml)

RC00007

PE6c

$150.00 30 µg (3mg/ml) 100 μg(10mg/ml) 500 μg(10mg/ml) 5 mg (10mg/ml)

RC00008

PE6d

RC00009

PE7

$220.00 30 µg (3mg/ml) 100 μg(10mg/ml) 500 μg (10mg/ml) 5 mg(10mg/ml)

Custom Cas Protein Service

With our years of expertise in Cas protein, Base editor and Prime editor production, we deliver tailored enzymes across RUO to cGMP—built to meet your exact editing goals. Backed by proprietary engineering platforms and rapid gene synthesis, we offer fast, reliable, high purity and comprehensive Cas protein production service tailored to your needs. Projects can be completed in as little as 2 weeks, with regulatory support for IND submissions. From early discovery to therapeutic development, our custom solutions accelerate your CRISPR pipeline.

95% success rate

With proprietary engineering platforms

Industrial-leading Speed

Production in as little as 2 weeks

Activity Assay Development

Established protocol developed in-house

High Purity

With dual-tag strategy

Service Details

Amount

Scalable from µg to g level

Tag

Strep and His dual-tag strategy for enhanced purity

QC Standard(default)

Purity >= 90%

Endotoxin < 10 EU/mg

Nuclease removal

Timeline

3-6 weeks

Optional services and QC

Activity Assay

Endotoxin < 1 EU/mg

Tag removal and purification

Interested in GMP grade customization service of Cas protein, Base Editor and Prime Editor? Check out our cGMP Cas Protein capability here

Interested in cGMP Cas proteins?

We offer cGMP Cas9 proteins off-the-shelf, as well as custom cGMP bacterial expression for a variety of CRISPR proteins.

Case Studies

The fidelity and editing efficiency of GenCRISPR eSpCas9 Protein were evaluated against wildtype Cas9 on Jurkat cells and primary T cells. GenCRISPR eSpCas9 exhibited a superior ability to reduce off-target edits.

Low off-target effects_Jurkat cells

Low off-target effects_ Primary T cells

GenCRISPR eSpCas9 exhibits comparable knockout and knock-in efficiency to the wildtype SpCas9 in both Jurkat cells and primary T cells.

High Knockout_ Jurkat cells and Primary T cell

High Knock-in_ Primary T cell

The GenCRIPSR™ Ultra eSpCas9 (mt) shows high batch-to-batch consistency, with no significant differences in cell viability and consistent knockout/knock-in efficiency across the three batches at any concentration.

KO at TRAC sites in HEK293T cells

GFP KI at RAB11A in Jurkat cells

GenCRISPR eSpCas9 exhibits lower off-target effects compared to HiFi Cas9 products from leading suppliers, while its knockout and knock-in efficiencies are equivalent to or exceed those of the leading suppliers.

Low off-target effects_HEK293 T cell

High Knockout efficiency_HEK293 T cell

High Knock-in efficiency_Primary T cell

WT SpCas9

GenCRISPR SpCas9 demonstrated high gene editing efficiency in both in vitro cleavage tests and HEK293T cells.

In vitro cleavage test

Gene knockout efficiency_HEK293 T cell

Gene knockout efficiency_293 T cells & Jurkat cells

GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency of three batches shows no significant difference.

GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency is equivalent to that of the leading supplier I.

Higher editing efficiency was observed for β2M knockout in Jurkat cells using GenCRISPR™ Ultra ErCas12a-3NLS, His-tag (Cat. No. RC00012) compared to Supplier A, as assessed by NGS and flow cytometry for gene and protein knockout.

Higher knock-in efficiency with triple knockout was observed in primary human T cells using GenCRISPR™ Ultra ErCas12a-3NLS, His-tag (Cat. No. RC00012) compared to all other suppliers’ products tested.

On-time delivery of Cas12 mutant with high purity, low endotoxin, and nondetectable DNase, presenting higher activity than wild type in in-vitro cleavage assay with in-house gRNA and validated protocol.

QC report

4.93mg/mL

0.648EU/mg

90%

94%

No detected

~5mg

Red arrow: Full-length dsDNA substrate Green arrow: Fragment-1 dsDNA Blue arrow: Fragment-2 dsDNA

Base editor

Gene editing efficiency in Jurkat cells using ABE8e RNP

High A to G conversion across different doses

ABE8e protein effectively knocks out B2M protein

Prime editor

Gene editing efficiency in HEK293T cells using PE7 RNP at 50 pmol

PE7 mediated targeted 5 bp deletions at the HEK3 locus in HEK293 cells

No significant impact on cell viability

Purification procedure

Ni columna

TEV protease cleavage

Ni column

Superdex 200 column

Q sepharose column

Superdex 200

Purity

>90%, by SDS-PAGE under reducing conditions.

Yield

1.6mg/L

22.03mg/L

12.3mg/L

See what researchers worldwide have achieved with our Cas proteins:

Testimonials

Jack Castelli

Fred Hutchinson Cancer Center

"The people at GenScript have been tremendously helpful in providing materials for our lab. We've been able to test novel CRISPR systems such as prime editing and PASSIGE in a timely manner thanks to their work. I would recommend their services for your editing needs."

MariaMorello Miravet

Karolinska Institutet

"We are very pleased with the editing efficiency using base editors from GenScript, we got up to 100% KO!"

Resources

CRISPR/Cas Nuclease Solutions

From Discovery to GMP – The Complete Toolkit for Next-Generation Gene Editing

Catalog mRNA, saRNA, ciriRNA, LNPs and ladders

Ready To Use RNA and LNPs– Empowering Discovery and Innovation

CRISPR RNP Handbook

Enabling RNP-mediated CRISPR gene editing and transforming life science research

GenCRISPR™ Gene Editing Solutions

All solutions you need for a successful CRISPR gene editing project

Protocol: Prime Editing in HEK 293T cells with RNP

Material, method, troubleshooting guidance, and case studies

Base Editing Protocol for Jurkat Cells RNP

Unlocking CRISPR: Advances in Base Editing & Prime Editing and Future Applications

Dr. Jiabao Lv, Sr. Protein Scientist

FAQs

GenScript offers a broad catalog of ready-to-use Cas nucleases in protein format, including Cas9, Cas12a, Cas13a, MAD7, Base Editors, and Prime Editors. These are available in both RUO (Research Use Only) and GMP-grade formats to support applications from early discovery to clinical manufacturing.

Yes. Our custom Cas protein service can engineer and produce nucleases precisely to your specifications—covering RUO through cGMP production. We deliver defined yield, purity, and activity, with guaranteed performance packages and production timelines as short as two weeks.

Our catalog products are ideal for widely used gene editing applications where validated, off-the-shelf options meet your needs.

Custom Cas proteins are recommended if you require unique formats, novel nucleases, enhanced fidelity, altered PAM specificities, or other custom specifications.

All Cas proteins undergo rigorous QC testing for purity, activity, and minimal off-target effects. GMP-grade nucleases are manufactured following current Good Manufacturing Practice (cGMP) guidelines and are compliant with FDA/EMA/PMDA/NMPA regulations.

Catalog products are available with rapid turnaround and global shipping. Custom Cas protein projects can be completed in as little as two weeks after sequence confirmation, depending on complexity and production scale.

Yes. We offer GMP-grade enzymes specifically designed to meet the high performance, traceability, and compliance requirements of therapeutic development—from preclinical through commercial production.

The catalog nucleases in our portfolio are licensed from the Broad Institute, providing access to validated products with streamlined IP pathways. For custom projects, our team can work with you to address licensing and regulatory considerations to minimize delays in your program.

Our scientists provide technical support, troubleshooting, and regulatory guidance, including data packages to support IND filing. We work as an extension of your team from concept to scale-up.

Our Cas nucleases are validated for a range of applications, including:

Targeted genome knockout and knock-in (e.g., via Cas9, Cas12a, MAD7)

Precision single base editing (ABE, CBE)

Prime editing for seamless corrections without the need for a DNA donor template

RNA editing (Cas13a)

Cell line engineering and therapeutic development

You can browse our catalog Cas proteins online for immediate online ordering, or contact our custom services team to discuss your specifications, timelines, and regulatory requirements.

Get in Touch with GenScript CRISPR Experts

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262