Educational guide
CMV pp65 Peptide Pool
PepMix™ Human CMV (pp65) US$572.00 Excluding tax and shipping fees In stock Description About PepMix™ Human CMV (pp65) The PepMix™ Human CMV (pp65) peptide pool offers a highly effective tool for studying immune responses to Human Cytomegalovirus (HCMV). The p
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PepMix™ Human CMV (pp65)
US$572.00
Excluding tax and shipping fees
In stock
Description
About PepMix™ Human CMV (pp65)
The PepMix™ Human CMV (pp65) peptide pool offers a highly effective tool for studying immune responses to Human Cytomegalovirus (HCMV). The peptides included in this pool represent T-cell epitopes that are recognized by CMV-specific CD4 and CD8 T-cells across a broad range of MHC contexts (see table below). These T-cells, when activated, typically produce pro-inflammatory cytokines such as interferon-gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor (TNF).
Pool of 138 peptides derived from a peptide scan (15mers with 11 aa overlap) through 65 kDa phosphoprotein (pp65) (Swiss-Prot ID: P06725) of Human Cytomegalovirus (HCMV) - strain AD169.
PepMix™ Peptide Pool Human CMV pp65 Applications
PepMix™ Peptide Pool Human CMV pp65 Quality Grades
HLA Specificty of PepMix™ Peptide Pool Human CMV pp65
PepMix™ Human CMV (pp65) - Specifications
Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
Purity: Development Grade: each peptide purified to > 70% (HPLC/MS) - (equivalent to an average purity of 85%)
Delivery Format: Freeze-dried in glass vial
Application(s): T-cell Immunity
Condition(s)/Topic(s): Control, Opportunistic infections, Cytomegalovirus disease, Infection
Standard Delivery Time: 2-5 days
Visit our webpage for more information on Peptide Tools to Study CMV
Custom Human CMV pp65 Peptides & Pools
About PepMix™ Peptide Pools
PepMix™ peptide pools are protein-spanning mixtures of overlapping peptides designed for a variety of immunological applications, including:
Antigen-specific T-cell stimulation in assays like ELISPOT, ICS, cell-mediated cytotoxicity, or proliferation assays.
In-vitro T-cell expansion.
Dendritic cell pulsing.Immune monitoring and profiling of cellular immune responses.T-cell epitope identification.
Standardization of T-cell assays.
Benefits of PepMix™ Peptide Pools
Guaranteed identity and purity for each peptide.
Certificate of Analysis (CoA) and HPLC-MS data provided.
High batch-to-batch consistency.
No false positive T-cell responses due to contaminating deletion peptides.
No toxic inhibition of T-cell responses thanks to stringent peptide purification.
Minimal endotoxin contamination through low bioburden processes.
Animal-derived component-free (ADCF) policy.
HLA-independent stimulation using antigen-spanning peptide pools.
Additional Insights into HCMV and pp65
Role of CMV pp65 in Viral Biology
The pp65 protein, a critical tegument protein of HCMV, plays an essential role in:
Viral replication by aiding the incorporation of viral proteins into the host cell.
Modulating the host cell’s immune responses to ensure effective immune evasion.
Regulating HCMV-encoded genes during viral genome expression.
Research Applications
Understanding the role of cellular proteins and HCMV genes in the context of pp65 provides insights into:
The mechanisms of immune evasion by HCMV.
Development of antiviral strategies targeting pp65.
Broader implications for studying opportunistic infections in immunocompromised individuals.
References
References for PepMix™ Human CMV (pp65)
ReferencesRead References with PepMix™ Peptide Pools for Infectious Diseases COVID-19 related PepMixes™ PepMix™ Control Pools
Application NotesCross-reactive T cells enhance immune responses in SARS-CoV-2 infection and vaccinationLoyal et al (2021) Full textPeptide Tools to Support the Fight against COVID-19Alem et al (2020) Full textCEFX/EFX Ultra SuperStim Pools: Superior positive controls for T-cell assays with broad MHC-allele and antigen coverageEckey et al. (2017) Full textDeveloping Multi-HIV Antigen Specific T-Cells as a Component of a Cure StrategyLam et al. (2015) Full textPeptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell TransplantationGary et al. (2015) Full textPepMix™ Peptide Pools for Clinical Applications: T Cell Therapy for Viral Infections after Hematopoietic Stem Cell TransplantKeirnan et al. (2012) Full textCytomegalovirus Protein Spanning PepMix™ Peptide Pools to Discover Changes in T-Cell Immunity in the Aging PopulationKern (2012) Full text
Testimonials "To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our go-to company for purchasing peptides." Dr. Laura J Pallett, Infection and Immunity, University College London, UK
Documentation
Documentation for PepMix™ Human CMV (pp65)
Protocol_PepMix.pdf
PM-CMV-pp65-1.pdf
Properties
Properties of PepMix™ Human CMV (pp65)
25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
T-cell immunity
PepMix Peptide Pools
Control, Cytomegalovirus disease, Infection, Opportunistic infections
Freeze-dried in glass vial
Human Cytomegalovirus (HCMV)
65 kDa phosphoprotein (pp65)
No
Further Information to PepMix™ Human CMV (pp65)
Values
MESRGRRCPEMISVLGPISGHVLKAVFSRGDTPVLPHETRLLQTGIHVRVSQPSLILVSQYTPDSTPCHRGDNQLQVQHTYFTGSEVENVSVNVHNPTGRSICPSQEPMSIYVYALPLKMLNIPSINVHHYPSAAERKHRHLPVADAVIHASGKQMWQARLTVSGLAWTRQQNQWKEPDVYYTSAFVFPTKDVALRHVVCAHELVCSMENTRATKMQVIGDQYVKVYLESFCEDVPSGKLFMHVTLGSDVEEDLTMTRNPQPFMRPHERNGFTVLCPKNMIIKPGKISHIMLDVAFTSHEHFGLLCPKSIPGLSISGNLLMNGQQIFLEVQAIRETVELRQYDPVAALFFFDIDLLLQRGPQYSEHPTFTSQYRIQGKLEYRHTWDRHDEGAAQGDDDVWTSGSDSDEELVTTERKTPRVTGGGAMAGASTSAGRKRKSASSATACTSGVMTRGRLKAESTVAPEEDTDEDSDNEIHNPAVFTWPPWQAGILARNLVPMVATVQGQNLKYQEFFWDANDIYRIFAELEGVWQPAAQPKRRRHRQDALPGPCIASTPKKHRG Copy Sequence Show complete Sequence Show trimmed Sequence
Peptide scan (15mers with 11 aa overlap)
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