CMV (pp65) Human Peptide Pool | PepMix™

PepMix™ Human CMV (pp65) - Premium Grade

US$686.40

Excluding tax and shipping fees

In stock

Description

About PepMix™ Human CMV (pp65) - Premium Grade

Pool of 138 peptides derived from a peptide scan (15mers with 11 aa overlap) through 65 kDa phosphoprotein (pp65) (Swiss-Prot ID: P06725) of Human Cytomegalovirus (HCMV) - strain AD169.

PepMix™ Human CMV (pp65) peptide pools are essential tools for studying the immune response to CMV pp65, a major Cytomegalovirus protein critical in viral pathogenesis and immune recognition. This unique peptide pool stimulates T-cell responses, targeting both CD4 and CD8 T-cells across diverse MHC contexts (refer to table). These activated T-cells typically release pro-inflammatory cytokines, including interferon-gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor (TNF), which play critical roles in modulating immune defenses against hCMV infection.

PepMix™ Human CMV (pp65) - Premium Grade - Specifications

Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)

Purity: Premium Grade: each peptide purified to >90% (HPLC/MS) - (equivalent to an average purity of 95%)

Delivery Format: Freeze-dried in glass vial

Application(s): T-cell Immunity

Condition(s)/Topic(s): Control, Opportunistic infections, Cytomegalovirus disease, Infection

Standard Delivery Time: 2-5 days

Visit our webpage for more information on Peptide Tools to Study CMV

The Role of CMV pp65 in hCMV Infection and Host Immunity

The pp65 protein, a prominent tegument protein in Human Cytomegalovirus (HCMV), plays an integral role in viral replication, immune evasion, and host cell infection. As a key structural protein, it facilitates the assembly of viral particles, modulates viral gene expression, and impacts the virus-host interaction during the early stages of infection.

CMV pp65 is critical in shaping the immune landscape, acting as a dominant target for both innate immunity and adaptive T-cell responses. Its ability to stimulate infected cell recognition makes it an invaluable tool for studying viral genome expression and the immune surveillance mechanisms in various cell types.

PepMix™ Peptide Pool Human CMV pp65 Applications

The PepMix™ Human CMV (pp65) antigen detects T-cell responsiveness to HCMV. As most people have CMV-specific T-cells recognizing HCMV pp65 the PepMix™ Human CMV (pp65) is often used as a positive control in T-cell stimulation assays. It is also useful for isolating CD4 and CD8 T-cells from PBMCs after activation. This isolation uses antibody-labeled magnetic beads. HCMV pp65-specific T-cells can be enriched through peptide stimulation and proliferation, creating T-cell lines or clones. JPT’s PepMix™ Peptide Pool Human CMV (pp65) is suitable for various cell assays needing high-quality peptides, including cytokine production-dependent assays like ELISPOT or flow cytometry, and detection of surface activation markers such as anti-CD154 or anti-CD137. Unlike non-specific controls such as as staphylococcus enterotoxin B (SEB), phorbol-12-myristate 13-acetate(PMA)/Ionomycin, or phytohemagglutinin (PHA), PepMix™ provides a T-cell receptor-dependent activation control, making it a more physiological and relevant option for peptide-based T-cell activation assays.

HLA Specificty of PepMix™ Peptide Pool Human CMV pp65

Peptides from the CMV pp65 protein are known to be presented by a wide range of HLA-molecules (note that some studies reported low-resolution, serologic specificities whereas others reported specific alleles). JPT can provide individual T-cell stimulating CMV-derived pp65 peptides for each mentioned allele. MHC molecules presenting peptides derived from human CMV-pp65 (see table).

Applications of PepMix™ Human CMV (pp65)

Detection and Functional AnalysisThe PepMix™ Human CMV (pp65) antigen is a powerful tool for:

Detecting T-cell responsiveness to HCMV, a common issue during HCMV infection.

Using as a positive control in T-cell stimulation assays due to its ability to stimulate CMV-specific T-cells effectively.

Cytokine production-dependent assays, including ELISPOT and flow cytometry

Detection of surface activation markers such as anti-CD154 or anti-CD137.

Custom Solutions for Specialized Research

Looking for a tailored solution? Custom PepMix™ peptide pools and individual HLA-specific peptides provide flexibility for:

Exploring novel host-virus interactions in hCMV infection.

Standardizing T-cell assays for cross-laboratory consistency.

Developing advanced tools for immune monitoring in diverse patient cohorts.

Advancing Research on hCMV Genes and T-cell Responses

Harnessing the versatility of PepMix™ Human CMV pp65 enables researchers to:

Decode interactions between hCMV replication and host cells.

Study viral genome dynamics in infected and wild-type cells.

Identify and characterize T-cell epitopes for vaccine development and therapeutic interventions.

Benefits of PepMix™- Each peptide quality controlled and guaranteed for identity and purity- CoA and HPLC-MS data available- High batch-to-batch consistency- No false positive T cell responses by contaminating deletion peptides- No toxic inhibition of T cell responses due to stringent purification of each peptide- Minimization of endotoxin contamination due to low bioburden process- ADCF policy in place- HLA independent stimulation (with antigen spanning peptide pools)

Unique Benefits of PepMix™ for hCMV Research

PepMix™ peptide pools stand apart for their unmatched reliability and precision in T-cell receptor-dependent activation. Key benefits include:

HLA-independent stimulation, ensuring broader applicability across MHC molecules.

Superior batch-to-batch consistency for reproducible results.

Minimal endotoxin contamination, preserving innate immunity response fidelity.

Fully documented quality assurance, including CoA and HPLC-MS data.

References

References for PepMix™ Human CMV (pp65) - Premium Grade

ReferencesRead References with PepMix™ Peptide Pools for Infectious Diseases COVID-19 related PepMixes™ PepMix™ Control Pools

Application NotesCross-reactive T cells enhance immune responses in SARS-CoV-2 infection and vaccinationLoyal et al (2021) Full textPeptide Tools to Support the Fight against COVID-19Alem et al (2020) Full textCEFX/EFX Ultra SuperStim Pools: Superior positive controls for T-cell assays with broad MHC-allele and antigen coverageEckey et al. (2017) Full textDeveloping Multi-HIV Antigen Specific T-Cells as a Component of a Cure StrategyLam et al. (2015) Full textPeptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell TransplantationGary et al. (2015) Full textPepMix™ Peptide Pools for Clinical Applications: T Cell Therapy for Viral Infections after Hematopoietic Stem Cell TransplantKeirnan et al. (2012) Full textCytomegalovirus Protein Spanning PepMix™ Peptide Pools to Discover Changes in T-Cell Immunity in the Aging PopulationKern (2012) Full text

Testimonials "To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our go-to company for purchasing peptides." Dr. Laura J Pallett, Infection and Immunity, University College London, UK

Documentation

Documentation for PepMix™ Human CMV (pp65) - Premium Grade

Protocol_PepMix.pdf

PM-CMV-pp65-Premium-Grade-2.pdf

Properties

Properties of PepMix™ Human CMV (pp65) - Premium Grade

25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)

T-cell immunity

PepMix Peptide Pools

Control, Cytomegalovirus disease, Infection, Opportunistic infections

Freeze-dried in glass vial

Human Cytomegalovirus (HCMV)

65 kDa phosphoprotein (pp65)

No

Further Information to PepMix™ Human CMV (pp65) - Premium Grade

Values

MESRGRRCPEMISVLGPISGHVLKAVFSRGDTPVLPHETRLLQTGIHVRVSQPSLILVSQYTPDSTPCHRGDNQLQVQHTYFTGSEVENVSVNVHNPTGRSICPSQEPMSIYVYALPLKMLNIPSINVHHYPSAAERKHRHLPVADAVIHASGKQMWQARLTVSGLAWTRQQNQWKEPDVYYTSAFVFPTKDVALRHVVCAHELVCSMENTRATKMQVIGDQYVKVYLESFCEDVPSGKLFMHVTLGSDVEEDLTMTRNPQPFMRPHERNGFTVLCPKNMIIKPGKISHIMLDVAFTSHEHFGLLCPKSIPGLSISGNLLMNGQQIFLEVQAIRETVELRQYDPVAALFFFDIDLLLQRGPQYSEHPTFTSQYRIQGKLEYRHTWDRHDEGAAQGDDDVWTSGSDSDEELVTTERKTPRVTGGGAMAGASTSAGRKRKSASSATACTSGVMTRGRLKAESTVAPEEDTDEDSDNEIHNPAVFTWPPWQAGILARNLVPMVATVQGQNLKYQEFFWDANDIYRIFAELEGVWQPAAQPKRRRHRQDALPGPCIASTPKKHRG Copy Sequence Show complete Sequence Show trimmed Sequence

Peptide scan (15mers with 11 aa overlap)

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