Educational guide
Assessing Peptide Stability Under Thermal Stress
ARGEN, by Fluence Analytics, offers scientists the instruments they need to effortlessly surveil changes in the normalized molecular weight of peptide samples in solution. It facilitates the identification of aggregation onset, degradation, or phase changes as
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ARGEN, by Fluence Analytics, offers scientists the instruments they need to effortlessly surveil changes in the normalized molecular weight of peptide samples in solution. It facilitates the identification of aggregation onset, degradation, or phase changes as these phenomena occur due to thermal, stir, pH, ionic strength, and other stressors.
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Study: Comparing peptide stability under similar stressors
Two peptide samples from distinct production lots were exposed to comparable stressors to identify which had a higher propensity for aggregation and inherent instability.
Method and stressors:
The researchers prepared six samples of each peptide lot at 1mg/ml in two pH solutions, 2.5 and 3.0. Three of the six solutions were next exposed to thermal stress of 18C, 25C and 35C alone while the remaining samples were exposed to thermal stress and stirring stress at 300RPM.
Image Credit: Yokogawa Fluence Analytics
Analysis:
The following graph demonstrates the quantified Aggregation Rates for all peptides with and without stirring and at various temperatures and pH levels. The data suggests that peptide Lot 1 is more stable than Lot 2 when exposed to conditions of thermal and stirring stress. Changing the solution pH from 3.0 to 2.5 induced an extra stability gain of around 10x reduction in the AR value.
Image Credit: Yokogawa Fluence Analytics
Image Credit: Yokogawa Fluence Analytics
Image Credit: Yokogawa Fluence Analytics
Image Credit: Yokogawa Fluence Analytics
Image Credit: Yokogawa Fluence Analytics
About Yokogawa Fluence Analytics
Yokogawa Fluence Analytics, which was named as a Top 50 global advanced manufacturing startup by CB Insights, provides patented process analytics and control solutions to polymer and biopharmaceutical customers worldwide. Yokogawa Electric Corporation acquired Fluence Analytics in January 2023.
Yokogawa Fluence Analytics is a global leader in real-time polymer reaction monitoring and control, and its industry-leading ACOMP product is the only commercially available smart manufacturing system that continuously monitors and measures polymerization reactions.
The company’s biopharmaceutical product line includes a high-throughput static light scattering instrument called ARGEN. ARGEN can independently measure the stability of biopolymers under thermal, chemical, and mechanical (physical) stress, while also performing shelf-life stability studies at low temperatures.
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