AOD-9604 Protocol — Complete Dosing & Administration Guide

Dosing

How muchdo I take?

Subcutaneous: A small injection into the fatty layer just under the skin — the same way insulin is given.

Bioavailability High — most of the dose reaches your bloodstream, just more gradually than an IV.

Starting Dose

250 mcg

Frequency

Once daily

Duration

2-4 weeks

AOD9604 is a research peptide and not an approved medicine. Doses around 250 mcg under the skin are a common starting point in practice. In a human trial the most effective amount was about 1 mg per day. [1][5]

Standard Dose

300-400 mcg

8-12 weeks

A commonly used daily amount in practice. The largest human study found benefit up to about 1 mg/day with no extra gain from higher doses. [1][5]

Advanced Dose

500 mcg

A higher amount sometimes used in practice. Going above roughly 1 mg/day did not improve results in the human trial. [1][5]

Timing

Best time to take

Morning on an empty stomach, 30-60 minutes before food or exercise

With food?

Best taken on an empty stomach for optimal absorption. Wait at least 30 minutes before eating.

If stacking

Can be combined with GHRP peptides like Ipamorelin at the same time. If using with CJC-1295, inject together for synergistic effects on fat metabolism.

Adjusting Your Dose

Increase if

+Tolerating current dose well with no side effects after 2-3 weeks

+Fat loss progress has stalled despite consistent diet and exercise

+Seeking enhanced fat oxidation during intensive training phases

Decrease if

-Experiencing persistent headaches or flushing

-Injection site reactions are bothersome

-Any signs of allergic reaction occur

Signs of right dose

✓Gradual reduction in body fat, especially in stubborn areas

✓No significant side effects

✓Improved body composition without muscle loss

✓Stable energy levels throughout the day

Dosing Calculator

Calculate Your Exact Dose

Step 1: Peptide Weight

Find the weight printed on your peptide vial label

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The peptide weight is printed on the label

The weight is on the label

Select Weight

Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Administration

How do Iuse it?

Reconstitution

What you need

•Bacteriostatic water (BAC water)

•Insulin syringe (29-31 gauge)

•Alcohol swabs

•Sterile AOD-9604 vial (typically 5mg)

Example

5mg vial + 2mL BAC water = 2.5mg/mL (2500mcg/mL). For 250mcg dose, draw 0.1mL (10 units on insulin syringe).

At 2500mcg/mL concentration: 0.1mL = 250mcg, 0.15mL = 375mcg, 0.2mL = 500mcg

Injection

Route

Subcutaneous injection into fatty tissue

Best sites

•Abdomen (2 inches from navel)—most common site for fat-targeting

•Love handle area—some prefer injecting near target fat areas

•Outer thigh (middle third)

Technique

1.Clean injection site thoroughly with alcohol swab and let dry

2.Pinch a fold of skin between thumb and forefinger

3.Insert needle at 45-90 degree angle into the pinched fold

4.Inject slowly and steadily

5.Remove needle and apply light pressure—do not rub

Storage

Before reconstitution

Refrigerate at 36-46°F (2-8°C) for up to several months. Can also be stored frozen at -4°F (-20°C) for longer-term storage. Keep in original packaging away from light.

After reconstitution

Refrigerate immediately at 36-46°F (2-8°C). Never freeze after mixing with bacteriostatic water. Store away from light. Discard after 28 days or if any degradation signs appear.

Signs of degradation

Cloudy or milky appearance (should be clear)

Visible particles or floaters in solution

Unusual color change

Clumping or precipitation at bottom of vial

Sample Daily Schedule

Morning (upon waking, 6-8 AM)

250-500mcg injection

Site: Abdomen or target fat area

Inject on empty stomach. Wait 30-60 minutes before eating. Best before fasted cardio if exercising.

Alternative: Before bed (optional split dose)

250mcg injection

Site: Abdomen or outer thigh

If splitting a 500mcg daily dose, take second injection before bed on empty stomach. Allow 3+ hours after last meal.

Safety

Is itsafe?

Safety Profile

AOD-9604 has been studied in multiple human clinical trials involving thousands of participants. The safety profile is notably clean—it doesn't affect blood sugar, insulin levels, or cause the typical side effects of growth hormone. Phase 2 trials showed it was well-tolerated even at doses higher than typically used. The most common side effects are mild and injection-site related.

Metabolic Pharmaceuticals invested over $50 million in research, generating extensive human safety data. Multiple Phase 1-2 trials demonstrated excellent tolerability. The compound received GRAS (Generally Recognized as Safe) status for oral use in some jurisdictions. However, Phase 3 trials for obesity were not completed.

Common Side Effects

Experienced by some users

Injection site reactions

Redness, minor swelling, or tenderness at the injection site that typically resolves within hours

Management: Rotate injection sites, ensure proper technique, apply ice if needed

Mild headache

Occasional headache, especially in the first few days of use

Management: Stay well hydrated, usually resolves with continued use

Flushing or warmth

Temporary flushing sensation after injection, lasting minutes

Management: This is normal and harmless—resolves quickly on its own

Less Common

•Fatigue

•Dizziness

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

×Signs of allergic reaction (rash, swelling, difficulty breathing)

×Persistent side effects that don't improve after dose reduction

×Pregnancy confirmed or suspected

×Development of any new medical condition—consult your provider

×No noticeable benefits after 8-12 weeks of consistent use

×Prior to any surgical procedure—discuss timing with your surgeon

Always consult with a healthcare provider before stopping or if you experience any concerning symptoms. This information is for educational purposes and should not replace professional medical advice.

Interactions

With other peptides

✓Excellent synergy for fat loss—can be injected together

✓Commonly stacked for body recomposition—complementary mechanisms

✓Safe to combine; different mechanisms, no interaction concerns

✓Safe combination for those seeking fat loss plus recovery benefits

!Generally not combined—AOD-9604 mimics part of HGH's action

With medications

✓Insulin - AOD-9604 does not affect blood sugar, but monitor if diabetic

✓Diabetes medications - No known interactions, but monitor blood sugar as always

✓Blood thinners - Use caution—may slightly increase bruising at injection sites

✓Thyroid medications - No known interactions, safe to use together

With supplements

✓L-Carnitine - May enhance fat oxidation effects—good synergy

✓Caffeine/Thermogenics - Safe to combine for enhanced fat loss, but not necessary

✓Fish oil/Omega-3s - Safe and may support overall metabolic health

✓Protein supplements - Recommended to support muscle preservation during fat loss

Want the Full Picture?

View the complete AOD-9604 research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.