Educational guide
Antibody Services | JPT Peptides
Antibody Services Peptide Antibody Services Immunizing with a peptide corresponding to a specific region of a protein antigen is a popular method to develop custom antibodies. With this strategy, you only need the amino acid sequence of a predicted/selected ep
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Antibody Services
Peptide Antibody Services
Immunizing with a peptide corresponding to a specific region of a protein antigen is a popular method to develop custom antibodies. With this strategy, you only need the amino acid sequence of a predicted/selected epitope within the protein antigen, avoiding homologous regions to minimize cross reactivity with other proteins.
Antigenic peptides of 10-20 amino acids in length provide optimal epitope diversity while still offering superior specificity compared to immunizing with the full length protein sequence. The peptide is usually conjugated to a carrier protein such as KLH or BSA to enhance stimulation of an immune response. Antibodies are generated against the peptide sequence and the carrier protein. Therefore, the highly specific antibodies directed against the peptide are separated by affinity purification.
Why Use Peptide Antigens for Immunization?
Chemical synthesis yields defined epitopes
Low probability of cross-reactivity
High flexibility in antigen selection
Sequence diversity and PTMs can be addressed
Easy to synthesize, cost-effective and stable
Applications for Antibody Services
Antigen design
Production of antigen conjugates
Titer determination
Hybridoma screening
Epitope mapping
Blocking peptides
JPT's Antibody Peptide Formats
Filter products
Assay Services
B-cell Immunity
Cancer biomarker research
Enzymology
Nuclear medicine
Protein-protein-interaction
Proteomics
Custom Peptides
Enzyme Substrate Arrays
Micro-Scale Peptides
PTM Peptide Reference Standards
PepStar Peptide Microarrays
Histones
BioTides Biotinylated Peptides
Custom PepStar™ Peptide Microarrays
Peptide Conjugates - KLH, BSA
Peptide ELISA
Peptide Modifications & Speciality Peptides
Antibody Epitope Discovery Service
Comprehensive Solutions for Post-Translational Modifications (PTMs)
Histone Antibody Specificity Profiling
Multiwell Microarray Service
Immunogen Design
Design and production of immunogenic peptides (e.g. KLH, BSA, OVA conjugates or MAP –multiple antigenic peptides)
Modified peptides carrying PTMs such as phosphorylation, citrullination, acetylation and more
Design supported by data mining, modeling and bioinformatics
Assay Development
Screening tools such as pre-coated peptide ELISA plates for fast and easy titer determination without the need of carrier conjugation
B-Cell ELISpot (total IgG and peptide-specific)
Identification of soluble biomarkers as target for antibody development
Antibody Development
Sophisticated peptide microarrays improve and accelerate your hybridoma screening
Antibody epitope mapping service using our proprietary PepStar™ peptide microarray technology
Specificity testing of antibodies (including PTMs)
SAR for antibody/peptide interactions
Design and production of mimotopes and biosimilars
Process Optimization
Peptides as assay control
Affinity Purification
References
Expression of Programmed Death Ligand (PD-L1) in Different Tumors. Comparison of Several Current Available Antibody Clones and Antibody ProfilingKintsler et al., Annals of Diagnostic Pathology (2019)
Der p 5 Allergen from House Dust Mite: First Epitope Mapping of Rabbit IgG Blocking AntibodiesLahiani et al., New Microbes and New Infections (2019)
Envelope-Specific Epitope Recognition Patterns of HIV Vaccine-Induced IgG Antibodies are Linked to Immunogen Structure and SequenceNadai et al, Front. Immunol (2019)
Development of a Novel Fully-Human Anti-CD123 Antibody to Target Acute Myeloid LeukemiaBaraniaket al, Leukemia Research (2019)
Array-In-Well Epitope Mapping of Phage-Displayed AntibodiesLamminmäki et al., Epitope Mapping Protocols (2018)
Analytical Performance of an Immunoassay to Measure ProenkephalinDonato et al, Clinical Biochemistry (2018)