Educational guide
AICAR Dosage Protocol | PeptideDosages.com
AICAR (50 mg Vial) Dosage Protocol AICAR Dosage Chart AICAR is dosed at 1 mg–3 mg daily via subcutaneous injection in educational protocols. A 50 mg vial reconstituted with bacteriostatic water yields about 16.7 mg/mL. This information is for research and educ
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AICAR (50 mg Vial) Dosage Protocol
AICAR Dosage Chart
AICAR is dosed at 1 mg–3 mg daily via subcutaneous injection in educational protocols. A 50 mg vial reconstituted with bacteriostatic water yields about 16.7 mg/mL. This information is for research and educational use only.
Reconstitute: Add 3.0 mL bacteriostatic water → ~16.7 mg/mL concentration.
Research dosing range: 1,000–3,000 mcg once daily (conservative gradual approach).
Easy measuring: At 16.7 mg/mL, 1 unit = 0.01 mL ≈ 167 mcg on a U-100 insulin syringe.
Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 4 weeks; avoid freeze–thaw cycles.
AICAR (5-aminoimidazole-4-carboxamide ribonucleoside) is a research compound that activates AMP-activated protein kinase (AMPK)[1], earning the nickname “exercise pill” after studies showed sedentary animals given AICAR experienced dramatic endurance improvements[2]. AICAR is prohibited in sports (WADA classifies it as a metabolic modulator) and is not approved for therapeutic use in humans[1]. This educational protocol presents a once-daily subcutaneous approach for research purposes only.
Related research: For distinct compound, component, or formulation evidence and safety context, read MOTS-c Peptide: Benefits, Uses, Side Effects, Dosage, and Research. These links are comparisons only; the compounds and formulations should not be treated as interchangeable.
Standard / Gradual Approach (3 mL = ~16.7 mg/mL)
Weeks 1–2
1,000 mcg (1 mg)
6 units (0.06 mL)
Weeks 3–4
2,000 mcg (2 mg)
12 units (0.12 mL)
Weeks 5–8
3,000 mcg (3 mg)
18 units (0.18 mL)
Frequency: Inject once daily subcutaneously[3]. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability.
Reconstitution Steps
Draw 3.0 mL bacteriostatic water with a sterile syringe.
Inject slowly down the vial wall; avoid foaming.
Gently swirl/roll until dissolved (do not shake).
Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Advanced Protocol (Higher Doses)
Weeks 3–6
Weeks 7–12
5,000 mcg (5 mg)
30 units (0.30 mL)
Note: Higher-dose protocols should only be considered when explicitly supported by literature[4]. Research doses remain well below human safety studies (up to 210 mg/kg IV)[5], providing a wide safety margin.
Supplies Needed
Plan based on an 8-week research protocol (conservative dosing).
Peptide Vials (AICAR, 50 mg each):
8 weeks (gradual 1–3 mg/day) ≈ 3 vials
12 weeks (2–5 mg/day advanced) ≈ 8 vials
Insulin Syringes (U-100, or 30/50-unit for precision):
Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.
8 weeks (3 vials): 9 mL → 1 × 10 mL bottle
12 weeks (8 vials): 24 mL → 3 × 10 mL bottles
Alcohol Swabs: One for the vial stopper + one for the injection site each day.
Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100-count boxes
12 weeks: 168 swabs → recommend 2 × 100-count boxes