Independent education resourceInformation here does not replace care from a qualified health professional.
Peptide Therapy GuideClear peptide education

Educational guide

A FLASH of Possibilities.

A FLASH of Possibilities. No Twists. No Tricks. 4 Days from Gene to Plasmid Home » Gene Synthesis & Molecular Biology Services » FLASH Gene & Gene Synthesis - 4-Day Gene to Plasmid FLASH Gene is Changing the Equation of Gene Synthesis 4-Business-Day Turnaround

Written by Peptide Therapy Guide Editorial Team
For education only

This guide cannot diagnose a condition or recommend a personal treatment plan. Discuss medical questions with a qualified professional.

A FLASH of Possibilities.

No Twists. No Tricks.

4 Days from Gene to Plasmid

Home » Gene Synthesis & Molecular Biology Services » FLASH Gene & Gene Synthesis - 4-Day Gene to Plasmid

FLASH Gene is Changing the Equation of Gene Synthesis

4-Business-Day Turnaround – Speed, no rush fees.

3-in-1: Gene to Plasmid – Accuracy, no exceptions.

Flat Pricing – Clarity, no surprises.

Researchers around the world are making the switch—why not you?

Order today and experience A FLASH of Possibilities. No Twists. No Tricks.

FLASH Gene — 4 BD Gene Synthesis

FLASH Gene — Shipped in just 4 business days, powered by the revolutionary GenGenius™ automation platform, starting at $89 flat rate that includes: DNA synthesis, cloning, and default 4µg plasmid prep. No hidden fees, just fast, transparent, and seamless results to accelerate your research!

See FLASH Gene Pricing Table.

How to Order Gene Synthesis via GenSmart™ 2.0

Upload yourgene sequence

Choose from GenScript’sFLASH Gene Vectors170+ Available

Select your plasmidpreps quantity andquality

Sequence perfect,guarantee yield genes shipped to you

Need more than 3 kb genes? We offer customized solutions up to 200 kb (Check out GenBrickTM) Visit our FAQ.

Cloning Vectors

Mammalian Expression Vectors

Bacterial Expression Plasmids

Yeast Expression Plasmids

Viral Vector

Antibody Expression

pBluescript II KS(-)

pcDNA3.1(-)

pGEX-2TK

pET-9a

pET-30a(+)

pAO815

pAcSG2

pAb-HC-hIgG1

pBluescript II KS(+)

pcDNA3.1(-)-myc-His A

pGEX-4T-1

pET-11a

pET-30b(+)

pESC-HIS

pBAC-1

pAb-LC-hkappa

pBluescript II SK(-)

pcDNA3.1(-)-myc-His B

pGEX-4T-1-H(RBS)

pET-11b

pET-30c(+)

pESC-LEU

pBacPAK8

pAb-LC-hlambda

pBluescript II SK(+)

pcDNA3.1(-)-myc-His C

pGEX-4T-1-M(RBS)

pET-11c

pET-31b(+)

pESC-TRP

pBacPAK9

pAb-HC-hIgG2

pGenDONR

pcDNA3.1(+)

pGEX-4T-2

pET-11d

pET-32a(+)

pESC-URA

pFastBac1

pAb-HC-hIgG4

pJET1.2

pcDNA3.1(+)-C-6His

pGEX-4T-3

pET-14b

pET-32b(+)

pFastBac-Dual

pAb-HC-mIgG1

pUC18

pcDNA3.1(+)-C-DYK

pGEX-5X-1

pET-15b

pET-41a(+)

pFastBacHT-A

pAb-HC-mIgG2a

pUC19

pcDNA3.1(+)-C-DYK-P2A

pGEX-5X-1-H(RBS)

pET-16b

pET-41b(+)

pFastBacHT-B

pAb-HC-mIgG2b

pUC57

pcDNA3.1(+)-C-eGFP

pGEX-5X-1-M(RBS)

pET-17b

pET-41c(+)

pFastBacHT-C

pAb-LC-mkappa

pUC57-BsaI-BsmBI-free

pcDNA3.1(+)-C-HA

pGEX-5X-2

pET-19b

pET-42a(+)

pVAX1-Pro

pAb-LC-mlambda

pUC57-BsaI-free

pcDNA3.1(+)-C-Myc

pGEX-5X-3

pET-20b(+)

pET-42b(+)

pLVV-Empty

pAb-LC-mlambda2

pUC57-Kan

pcDNA3.1(+)-myc-His A

pGEX-6P-1

pET-21a(+)

pET-42c(+)

pLVV-CMV-PGK-BSD

pAb-HC-rabIgG

pUC57-Kan-BsmBI-free

pcDNA3.1(+)-myc-His B

pGEX-6P-1-H(RBS)

pET-21b(+)

pET-43.1a(+)

pLVV-CMV-PGK-Puro

pAb-LC-rabKappa

pUC57-Kan-Key IIS Free

pcDNA3.1(+)-myc-His C

pGEX-6P-1-M(RBS)

pET-21d(+)

pET-43.1b(+)

pLVV-CMV-P2A-Puro

pAb-LC-rabKappa2

pUC57-Kan-mini

pcDNA3.1(+)-N-6His

pGEX-6P-2

pET-22b(+)

pET-45b(+)

pLVV-CMV-PGK-BSD-P2A-EGFP

pAb-HC-ratIgG2a

pUC57-Kan-mini-BsmBI-Free

pcDNA3.1(+)-N-DYK

pGEX-6P-3

pET-23a(+)

pET-50b(+)

pLVV-CMV-PGK-Puro-P2A-EGFP

pAb-LC-ratkappa

pUC57-Kan-mini-Key IIS Free

pcDNA3.1(+)-N-DYK-P2A

pGS-21a

pET-24a(+)

pET-51b(+)

pLVV-EF1α-PGK-Puro

pAb-HC-hIgG1-hole

pUC57-Kan-SapI-free

pcDNA3.1(+)-N-eGFP

pMAL-c4x

pET-24a(+)-TEV

pET-52b(+)

pLVV-EF1α-PGK-Puro-P2A-EGFP

pAb-HC-hIgG1-knob

pUC57-Kan-Simple

pcDNA3.1(+)-N-GST(TEV)

pMAL-c4x-1-H(RBS)

pET-24b(+)

pETDuet-1

pAb-HC-hIgG1-hole(CH2-CH3)

pUC57-Kan-simple-BsmBI-Free

pcDNA3.1(+)-N-GST(Thrombin)

pMAL-c4x-1-M(RBS)

pET-24c(+)

pAb-VHH-hIgG1(CH2-CH3)

pUC57-Kan-Simple-Key IIS Free

pcDNA3.1(+)-N-HA

pMAL-c5E

pET-24d(+)

pAb-HC-hIgG4(S228P)

pUC57-Key IIS Free

pcDNA3.1(+)-N-Myc

pMAL-c5x

pET-25b(+)

pUC57-Mini

pcDNA3.1(+)-P2A

pMAL-p5E

pET-26b(+)

pUC57-Mini-BsaI-Free

pcDNA3.1(+)-P2A-eGFP

pMAl-p5g

pET-27b(+)

pUC57-Mini-Kana-BsmBI free-terminator-T7 deleted

pcDNA3.1/Hygro(-)

pMAl-p5x

pET-28a(+)

pUC57-Mini-Key IIS Free

pcDNA3.1/Hygro(+)

pQE-1

pET-28a(+)-TEV

pUC57-Simple

pcDNA3.1/Zeo (-)

pQE-60

pET-28b(+)

pUC57-Simple-BsaI-BsmBI-Free

pcDNA3.1/Zeo (+)

pET-3a

pET-28c(+)

pUC57-Simple-Key IIS Free

pcDNA3.1+/C-(K)-DYK

pET-3b

pET-29a(+)

pcDNA3.4

pET-3c

pET-29b(+)

pCI-Neo

pET-3d

pET-29c(+)

For more information include full vector sequence and cloning sites please visit Vector Library.

Customer Reviews — Case Studies

"I recently had the pleasure of using GenScript's Gene Synthesis service. I was impressed by their Fair, Transparent Pricing and Exceptional Customer Service, which included prompt and clear communication. They delivered my genes on schedule, earning my trust and recommendation for anyone in need of Reliable gene synthesis services."

Matt Wang

"GenScript's services, including Gene Synthesis, Peptide Synthesis, and Antibody Production, have proven invaluable to my research. Their services are always of the Highest Quality with Reliable turnaround times. "

Venkata Vamsee Mallajosyula

"GenScript simplified my Gene Synthesis order, quickly designing and creating exactly what I needed, even correcting my submission errors. Fast and reliable service! Expert staff!"

Maranda McDonald

"GenScript has been my go-to for DNA Cloning, Mutagenesis, and Gene Synthesis services. Consistently over the past several years, with hundreds constructs ordered, I have received excellent customer service, pricing, and speedy delivery without any letdowns."

Christopher Lennon

"Thanks to GenScript, I was able to generate a CAR-T cell product with high transduction efficiency and efficacy. I'm glad to have GenScript's help with gene synthesis and plasmid preparation in my workflow, and I plan to keep using their services for my future projects, even after my PhD. They are extremely communicative and thorough at every stage of my project without even having to ask, and their transparency and pleasantness is why I continue to come back to GenScript"

Samantha Chin

"From 2009, till today I was with GenScript. I never face any challenge. very easy and commendably service I received from here. so many time I have worked with difficult to expressed gene. Thanks to GenScript for each and every solution. I am happy to work with you for many more years. "

Parvinder Kaur

FLASH Gene — FAQ

Yes! GenScript's Premium Gene service provides highly customizable options for complex genes, including GC-rich regions, up to 200 kb. Get an instant quote GenSmart™ 2.0 Online Ordering system or consult our 24/7 experts for tailored solutions.

FLASH Gene offers ultra-fast 4-day delivery with flat-rate pricing. Premium Gene supports more complex and longer sequences. See below for detailed breakdown.

Turnaround Time

4 business days

5–9+ business days

10+ business days

Sequence Length

Up to 3k bp

3kb - 8kb

8kb - 200kb

Vectors

150+ FLASH Gene Vectors or Onboard your own

200+ Vectors or onboard your own

pUC57-brick or pCc1-brick vector

Pricing

Flat-rate pricing starting at $89/construct (includes cloning, plasmid prep)

Starting at $0.15/bp

Starting at $0.39/bp

Sequence Complexity

Covers most of your needs

Supports your advanced needs

Supports your long DNA sequences needs

FLASH Gene redefines the future of gene synthesis, offering the fastest turnaround time and a unique flat-rate pricing model. Powered by the GenGenius™ automation platform, FLASH Gene is shipped in just 4 business days without compromising quality.

The turnaround time indicates production time only. FLASH Genes are shipped overnight or via express shipping from local production facilities.

Our flat rate pricing makes budgeting easy and ensures cost-effective DNA synthesis. This model covers DNA synthesis, cloning, and default 4ug plasmid preparation — all for a fixed price starting at just $89 per construct. No surprises, just clear and predictable costs.

Our GenSmart™ 2.0 platform automatically checks sequence eligibility. If your sequence doesn’t qualify, we offer Premium Gene for more advanced synthesis needs. You can also use our free codon optimization tool to improve your sequence and increase the chance of qualifying for FLASH Gene.

Our industry-leading, free codon optimization tool adjusts your sequence using host-preferred codons to improve expression and increase eligibility for FLASH Gene. Try GenScript’s free GenSmart™ Codon Optimization Tool here.

Sequence Input

Length: Up to 200,000 bp

Format: Nucleotide or Amino acid sequences

Cloning Options

Vectors: Choose from GenScript Clone Vectors, Expression Vectors, or your Vectors

Cloning Methods: Choose from restriction cloning, or seamless cloning

Plasmid DNA Options(From 2 Business Days)

Quantity: Choose from 10µg to 2g

Supercoil: Choose from predominantly supercoil, ≥ 80% supercoil, or ≥ 90% supercoil

Endotoxin Level: Choose from ≤ 0.1 EU/µg, ≤ 0.01 EU/µg, ≤ 0.005 EU/µg, or not guarantee

Poly A guarantee add-on service

AAV ITR guarantee add-on service

Optional quality control tests and production processes

Turnaround Time Options

From 72 hours The Fastest Rocket From 5 Business Days Most Popular Premium From 9 Business Days Best Value Basic

Rocket Gene Synthesis

Premium Gene Synthesis

Basic Gene Synthesis

<1,500

3+

5+

9+

1,500-3,000

8+

10+

3,000-5,000

7+

14+

5,000-8,000

Request a quote

15+

19+

8,000-15,000

N/A

20+

15,000-20,000

>20,000

If additional plasmid prep is required, the turnaround time will increase based on the selected prep tier, starting at +2 business days. For details, visit our Plasmid DNA Preparation Service.

The standard quality control for FLASH Gene includes Sanger or NGS sequencing. Additional quality controls can be bundled with plasmid DNA preparation.

Vector onboarding ensures the fastest turnaround with ready-to-clone vectors. Even if your vector is already with us, it needs to be archived again for the quickest turnaround.

If you choose to onboard your own vector, please note that it will add 5 business days to the turnaround time. To speed up your project, we also offer over 170 FLASH Gene vectors for you to select from.

GenSmart™ 2.0 Tutorial Video

GenSmart™ 2.0 helps design, optimize, and quote your genes instantly, with built-in tools like free codon optimization.

Assess your genes & vectors to confirm their eligibility for FLASH Gene.

Optimize your sequence with the best-in-class codon optimization algorithm.

Choose from our FLASH Gene Vectors or onboard your own vectors.

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262

Listing price include gene synthesis, cloning, and plasmid DNA preparation. All price in USD.

1 - 800 bp

4 µg

$89

4 BD

801 – 1,500 bp

$139

1,501 – 2,300 bp

$189

6 BD

2,301 –3,000 bp

$339

> 3,000 bp

Quote

Simple Genes Only

Turnaround Time: Production time includes gene synthesis, cloning, and 4 ug plasmid prep

Plasmid: Bundle with plasmid preparation service for flexible QC options (starting from 2 BD)

100% Sequence Accuracy Guaranteed

Vectors: Choose from 170+ GenScript FLASH Gene Vectors or Onboard Your Own Vector

Custom Vector: Vector evaluation and onboarding are required before project initiation (FLASH Gene Vector onboarding fee $50).

Application

For all-purpose use

Optimized for transfection studies

Optimized for animal studies

Turnaround time

From 2BD

From 3BD

From 10BD

Scale (Guarantee Yield)

≥10 µg

≥5 mg

To learn more about our QC options and add-on services, please visit Plasmid DNA Preparation Service

GenScript FLASH Gene Vector List

For more information include full vector sequence and cloning sites please visit https://www.genscript.com/expression-vector-selection-guide.html.

Typically, the synthesis success rate is mainly determined by DNA structures including repetitive sequences and GC content, and the stability in host cells including the toxicity for specific host cells. To improve the synthesis success rate of your gene sequences, we have summarized the main factors that we take into account for sequence analysis.

The main factors are including:

Global GC content less than 20% or higher than 80%

Various GC content within a DNA sequence

Homopolymers

Repeats of ≥ 20 bp

The number and the length of small repeats

Palindromic sequence

With over 18 years experience on nucleotide synthesis, GenScript has fully developed state-of-the-art platform to handle both easy and difficult gene sequences. For the coding sequence, we strongly recommend codon optimization to reduce the difficulty. If you have any questions on our Gene Synthesis Services, please contact your technical account manager for details.

Connected reading

Helpful context for this guide

Source-derived material selected through this article’s indexed topics.

Research context

Read sources and limitations before applying a claim.

Neuroscience Research Applications for Gene Synthesis

Resources » Technical Resource Centers » Gene Technical Resources » Applications for Gene Synthesis » Neuroscience Research Neuroscience Research Cancer Biology Genetic Engineering Vaccine Design Plant Biology Research

Source: genscript.com ↗
P

About the author

Peptide Therapy Guide Editorial Team

Editorial team for Peptide Therapy Guide.

View all articles →